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Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections

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Abstract

B cells and in particular antibodies has always played second fiddle to cellular immunity in regard to tuberculosis (TB). However, recent studies has helped position humoral immunity especially antibodies back into the foray in relation to TB immunity. Therefore, the ability to correlate the natural antibody responses of infected individuals toward TB antigens would help strengthen this concept. Phage display is an intriguing approach that can be utilized to study antibody-mediated responses against a particular infection via harvesting the B cell repertoire from infected individuals. The development of disease-specific antibody libraries or immune libraries is useful to better understand antibody-mediated immune responses against specific disease antigens. This study describes the generation of an immune single-chain variable fragment (scFv) library derived from TB-infected individuals. The immune library with an estimated diversity of 109 independent clones was then applied for the identification of monoclonal antibodies against Mycobacterium tuberculosis α-crystalline as a model antigen. Biopanning of the library isolated three monoclonal antibodies with unique gene usage. This strengthens the role of antibodies in TB immunity in addition to the role played by cellular immunity. The developed library can be applied against other TB antigens and aid antibody-derived TB immunity studies in the future.

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Acknowledgements

The authors would like to acknowledge the support from the Malaysian Ministry of Higher Education through the Fundamental Research Grant (FRGS) Scheme (grant no: 203/CIPPM/6711381) and Malaysian Ministry of Higher Education Higher Institution Centre of Excellence (HICoE) grant (grant no: 311/CIPPM/44001005). NMN acknowledges the support from the Malaysian Ministry of Higher Education through the Long-term Research Grant Scheme (LRGS) Scheme (grant no: 203/PPSK/67212002).

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Authors and Affiliations

  1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

    Nurul Hamizah Hamidon & Theam Soon Lim

  2. School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

    Siti Suraiya

  3. School of Heath Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia

    Maria E. Sarmiento, Armando Acosta & Mohd Nor Norazmi

  4. ADAPT Research Cluster, Centre for Research Initiatives – Clinical and Health Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Maria E. Sarmiento, Armando Acosta, Mohd Nor Norazmi & Theam Soon Lim

  5. Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

    Theam Soon Lim

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  1. Nurul Hamizah Hamidon
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Correspondence to Theam Soon Lim.

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Hamidon, N.H., Suraiya, S., Sarmiento, M.E. et al. Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections. Appl Biochem Biotechnol 184, 852–868 (2018). https://doi.org/10.1007/s12010-017-2582-5

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