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Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

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Abstract

Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L−1 in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L−1 total sugar) pretreated with activated charcoal and yeast extract (50 g.L−1). The HA produced exhibited a high molecular weight of 1.35 × 103 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L−1 was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid.

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Acknowledgements

The authors thank Coordination for the Improvement of Higher Education Personnel (CAPES – Brazil) for financial support, Dr. Dionisio Borsato from Londrina State University for support with the statistical analysis, and the Laboratory of Spectroscopy (SPEC) – State University of Londrina for the analyses.

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  1. Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina – UEL, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Caixa Postal 10.011, Londrina, PR, CEP 86.057-970, Brazil

    Nicole Caldas Pan, Hanny Cristina Braga Pereira & Maria Antonia Pedrine Colabone Celligoi

  2. Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista –UNESP, Rua Roberto Simonsen, n°. 305, Presidente Prudente, São Paulo, CEP 19060-900, Brazil

    Maria de Lourdes Corradi da Silva & Ana Flora Dalberto Vasconcelos

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  1. Nicole Caldas Pan
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Pan, N.C., Pereira, H.C.B., da Silva, M.d.L.C. et al. Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses. Appl Biochem Biotechnol 182, 276–293 (2017). https://doi.org/10.1007/s12010-016-2326-y

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