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6,6′-Bieckol Isolated from Ecklonia cava Protects Oxidative Stress Through Inhibiting Expression of ROS and Proinflammatory Enzymes in High-Glucose-Induced Human Umbilical Vein Endothelial Cells

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Abstract

Hyperglycemia-induced oxidative stress accelerates endothelial cell dysfunctions, which cause various complications of diabetes. The protective effects of 6,6′-bieckol (BEK), one of phlorotannin compound purified from Ecklonia cava against high-glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30 mM) treatment induced HUVECs’ cell death, but BEK, at concentration 10 or 50 μg/ml, significantly inhibited the high-glucose-induced cytotoxicity. Furthermore, treatment with BEK dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation, and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-kappa B (NF-κB) proteins in HUVECs, but BEK treatment reduced the overexpressions of these proteins. These findings indicate that BEK is a potential therapeutic agent that will prevent diabetic endothelial dysfunction and related complications.

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Acknowledgments

This research was also supported by the research programs of the Korea Institute of Ocean Science and Technology (PE99214). This research was supported by a grant from Marine Biotechnology Program Funded by Ministry of Oceans and Fisheries, Korea (PM 57770).

Author information

Authors and Affiliations

  1. Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, 617-736, Republic of Korea

    Mi-Hwa Park

  2. Global Bioresources Research Center, Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea

    Soo-Jin Heo

  3. Department of Marine Biology, University of Science and Technology, Daejeon, 305-350, Republic of Korea

    Soo-Jin Heo

  4. Department of Biotechnology, Konkuk University, Chungju, 380-701, Republic of Korea

    Pyo-Jam Park

  5. Division of Food Bioscience, and Korea Nokyong Research Center, Konkuk University, Chungju, 380-701, Republic of Korea

    Sang-Ho Moon, Si-Heung Sung, Byong-Tae Jeon & Seung-Hong Lee

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  1. Mi-Hwa Park
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Correspondence to Seung-Hong Lee.

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Mi-Hwa Park and Soo-Jin Heo contributed equally to this work.

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Park, MH., Heo, SJ., Park, PJ. et al. 6,6′-Bieckol Isolated from Ecklonia cava Protects Oxidative Stress Through Inhibiting Expression of ROS and Proinflammatory Enzymes in High-Glucose-Induced Human Umbilical Vein Endothelial Cells. Appl Biochem Biotechnol 174, 632–643 (2014). https://doi.org/10.1007/s12010-014-1099-4

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  • DOI: https://doi.org/10.1007/s12010-014-1099-4

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