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Isolation and Identification of α-Glucosidase and Protein Glycation Inhibitors from Stereospermum colais

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Abstract

Stereospermum colais (family Bignoniaceae) is a well-known pharmacologically potent medicinal plant reported in traditional systems of medicine. Phytochemical investigation of the roots of S. colais resulted in the isolation of seven compounds, and the metabolites were screened for its α-glucosidase enzyme inhibition and anti-glycation property. The compounds identified were β-sitosterol (1), 2-(4′-hydroxyphenyl) ethyl undecanoate (2), 2-(4′-hydroxyphenyl)ethyl pentadecanoate (3), 5α-ergosta-7,22-dien-3β-ol (4), ursolic acid (5), lapachol (6), and pinoresinol (7). Ursolic acid, lapachol, and pinoresinol possessed IC50 values of 119.01, 130.29, and 125.62 nM, respectively, compared to standard ascorbic acid with an IC50 value of 201.01 nM. The other compounds failed to show the activity. Results of the current study showcased the possible exploration of this medicinal plant for the treatment of type 2 diabetes in line with the development of phytopharmaceutical industry.

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References

  1. Kumar, G., & Murugesan, A. G. (2008). Hypolipidaemic activity of Helicteres isora L. bark extracts in streptozotocin induced diabetic rats. Journal of Ethnopharmacology, 116, 161–166.

    Article  CAS  Google Scholar 

  2. Matsui, T., Ueda, T., Oki, T., Sugita, K., & Terahara, N. J. (2001). α-Glucosidase inhibitory action of natural acylated anthocyanins. Journal of Agricultural and Food Chemistry, 49, 1948–1951.

    Article  CAS  Google Scholar 

  3. Dwek, R. A., Butters, T. D., Platt, F. M., & Zitzmann, N. (2002). Targeting glycosylation as a therapeutic approach. Nature Reviews in Drug Discovery, 1, 65–75.

    Article  CAS  Google Scholar 

  4. Lebovitz, H. E. (1997). α-Glucosidase inhibitors. Endocrinology and Metabolism. Endocrinology Metabolism Clinics of North America, 26, 539–551.

    Article  CAS  Google Scholar 

  5. Chopra, R.N., Nayar., S.L., Chopra, I.C. (1956) Glossary of Indian medicinal plants. New Delhi, p. 234.

  6. Vijaya Bharathi, R., Kumudha Veni, B., Jayashree, L., Suseela, M., & Thirumal, M. (2010). Antioxidant and wound healing studies on different extracts of Stereospermum colais leaf. International Journal of Research in Pharmaceutical Sciences, 1, 435–439.

    Google Scholar 

  7. Priya Rani, M., & Padmakumari, K. P. (2012). In vitro studies to assess the antidiabetic, antiperoxidative, and radical scavenging potential of Stereospermum colais. Pharmaceutical Biology, 50, 1254–1260.

    Article  Google Scholar 

  8. Apostolidis, E., Kwon, Y. I., & Shetty, K. (2007). Inhibitory potential of herb, fruit, and fungal enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Sciences and Emerging Technologies, 8, 46–54.

    Article  CAS  Google Scholar 

  9. Arom, J. (2005). In vitro antiglycation activity of arbutin. Naresuan University Journal, 13, 35–41.

    Google Scholar 

  10. Ratner, R. E. (2001). Controlling postprandial hyperglycemia. American Journal of Cardiology, 88, 26–31.

    Article  Google Scholar 

  11. Nathan, D. M. (2007). Finding new treatments for diabetes—how many, how fast…how good? The New England Journal of Medicine, 356, 437–440.

    Article  CAS  Google Scholar 

  12. Puls, W., Keup, U., Krause, H. P., Thomas, G., & Holffmeister, F. (1977). Glucosidase inhibition: a new approach to the treatment of diabetes, obesity and hyperlipoproteinemia. Naturwissenschaften, 64, 536–537.

    Article  CAS  Google Scholar 

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Acknowledgments

The first author thanks the Council of Scientific and Industrial Research for the award of Senior Research Fellowship and also thanks the director and head of the division for providing all the necessary facilities for carrying out the work. The authors also thank Ms. Saumini Mathew, Mr. Saran P. Raveendran, and Ms. S. Viji of CSIR-NIIST for recording NMR and mass spectra.

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Authors and Affiliations

  1. Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, Kerala, 695019, India

    M. Priya Rani, K. G. Raghu & K. P. Padmakumari

  2. Organic Chemistry Section, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, Kerala, 695019, India

    Mangalam S. Nair

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  1. M. Priya Rani
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  2. K. G. Raghu
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  3. Mangalam S. Nair
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  4. K. P. Padmakumari
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Correspondence to K. P. Padmakumari.

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Rani, M.P., Raghu, K.G., Nair, M.S. et al. Isolation and Identification of α-Glucosidase and Protein Glycation Inhibitors from Stereospermum colais . Appl Biochem Biotechnol 173, 946–956 (2014). https://doi.org/10.1007/s12010-014-0898-y

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  • DOI: https://doi.org/10.1007/s12010-014-0898-y

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