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RNAi Knockdown of Potent Sugar Sensor in Cellulase-Producing Fungus Acremonium cellulolyticus

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Abstract

A potent sugar sensor gene (g9105) was screened from the genomic data of the cellulase-producing fungus Acremonium cellulolyticus. The transcriptional level of g9105 in the SA49 transformant, which carried the knockdown RNA interference (RNAi) construct, was less than 10 % compared with the parental YP-4 strain. The hairpin-type RNAi construct could be useful for this fungal gene knockdown. Changes in cellulase productivity and protein secretion between these two strains were not observed. The numbers of hyphal tips at subapical branching site were counted for the SA49 and YP-4 strains incubated with potato-dextrose medium at 30 °C for 4 days. The hyphal branching ratio of the SA49 strain was higher than that of the YP-4 strain. The present results suggest that the potent sugar sensor gene in A. cellulolyticus could be related with hyphal branch formation.

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References

  1. Lynd, L. R. (1996). Annual Review Energy Environment, 21, 403–465.

    Article  Google Scholar 

  2. Ghose, T. K., & Sahai, V. (1979). Biotechnology and Bioengineering, 21, 283–296.

    Article  CAS  Google Scholar 

  3. Goyal, A., Ghosh, B., & Eveleigh, D. (1991). Bioresourse Technology, 36, 37–50.

    Article  CAS  Google Scholar 

  4. Yamanobe, T., Mitsuishi, Y., & Takasaki, Y. (1987). Agricultural and Biological Chemistry, 51, 65–74.

    Article  CAS  Google Scholar 

  5. Fang, X., Yano, S., Inoue, H., & Sawayama, S. (2009). Journal of Bioscience and Bioengineering, 107, 256–261.

    Article  CAS  Google Scholar 

  6. Kanna, M., Yano, S., Inoue, H., Fujii, T., & Sawayama, S. (2011). AMB Express, 1, 15.

    Article  CAS  Google Scholar 

  7. Fujii, T., Iwata, K., Murakami, K., Yano, S., & Sawayama, S. (2012). Bioscience, Biotechnology, and Biochemistry, 76, 245–249.

    Article  CAS  Google Scholar 

  8. Madi, L., McBride, S. A., Bailey, L. A., & Ebbole, D. J. (1997). Genetics, 146, 499–508.

    CAS  Google Scholar 

  9. Rappleye, C. A., Engle, J. T., & Goldman, W. E. (2004). Molecular Microbiology, 53, 153–165.

    Article  CAS  Google Scholar 

  10. Inoue, H., Fujii, T., Yoshimi, M., Taylor, L. E., II, Decker, S. R., Kishishita, S., et al. (2013). Journal Industrial Microbiology and Biotechnology, 40, 823–830.

    Article  CAS  Google Scholar 

  11. Ozcan, S., Dover, J., Rosenwald, A. G., Wölfl, S., & Johnston, M. (1996). Proceedings of the National Academy of Sciences of the United States of America, 93, 12428–12432.

    Article  CAS  Google Scholar 

  12. Momany, M. (2002). Current Opinion in Microbiology, 5, 580–585.

    Article  CAS  Google Scholar 

  13. Sahoo, D. K., Mishra, S., & Bisaria, V. S. (1986). Journal of General Microbiology, 132, 2761–2766.

    CAS  Google Scholar 

  14. Bisaria, V. S., Nanda, M., & Ghose, T. K. (1986). Journal of General Microbiology, 132, 973–978.

    CAS  Google Scholar 

  15. Trinci, A. P. J. (1973). Journal of General Microbiology, 81, 225–236.

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to Mr. Mitsutaka Nakata from Kyoto University for his helpful discussion. This study was supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan.

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Authors and Affiliations

  1. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, 606-8502, Japan

    Seiya Asada, Seiya Watanabe & Shigeki Sawayama

  2. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Seiya Watanabe

  3. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan

    Tatsuya Fujii, Hiroyuki Inoue & Kazuhiko Ishikawa

Authors
  1. Seiya Asada
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  2. Seiya Watanabe
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  3. Tatsuya Fujii
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  4. Hiroyuki Inoue
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  5. Kazuhiko Ishikawa
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  6. Shigeki Sawayama
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Corresponding author

Correspondence to Shigeki Sawayama.

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Seiya Asada and Seiya Watanabe equally contributed to this work

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Asada, S., Watanabe, S., Fujii, T. et al. RNAi Knockdown of Potent Sugar Sensor in Cellulase-Producing Fungus Acremonium cellulolyticus . Appl Biochem Biotechnol 172, 3009–3015 (2014). https://doi.org/10.1007/s12010-014-0728-2

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  • DOI: https://doi.org/10.1007/s12010-014-0728-2

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