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Preparation of New Calix[4]arene-Immobilized Biopolymers for Enhancing Catalytic Properties of Candida rugosa Lipase by Sol–Gel Encapsulation

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Abstract

The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol–gel supported by polycondensation with tetraethoxysilane and octyltriethoxysilane. The catalytic properties of immobilized lipase were evaluated into model reactions employing the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of naproxen methyl esters from racemic prodrugs in aqueous buffer solution/isooctane reaction system. The resolution studies using sol–gel support have observed more improvement in the enantioselectivity of naproxen E = 300 with Cel-Calix-E than with encapsulated lipase without calixarene-based materials. Furthermore, the encapsulated lipase (Cel-Calix-E) was still retained about 39 % of their conversion ratios after the fifth reuse in the enantioselective reaction.

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Acknowledgments

We would like to thank The Research Foundation of Selcuk University (BAP) for their financial support of this work.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Selcuk University, Konya, 42075, Turkey

    Elif Ozyilmaz & Serkan Sayin

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  1. Elif Ozyilmaz
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  2. Serkan Sayin
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Correspondence to Elif Ozyilmaz.

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Ozyilmaz, E., Sayin, S. Preparation of New Calix[4]arene-Immobilized Biopolymers for Enhancing Catalytic Properties of Candida rugosa Lipase by Sol–Gel Encapsulation. Appl Biochem Biotechnol 170, 1871–1884 (2013). https://doi.org/10.1007/s12010-013-0308-x

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