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Studies of Ciprofloxacin Encapsulation on Alginate/Pectin Matrixes and Its Relationship with Biodisponibility

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Abstract

Screening of ciprofloxacin (Cip) with selected biopolymers brings about 90% antibiotic interactions with a coacervate composed of alginate/high metoxylated pectin in 2:1 ratio. Fourier transform infrared spectroscopy analysis provides information about the nature of this interaction, revealing ionic and hydrophobic patterns among the molecules. Alginate/high methoxylated pectin gel microspheres developed by ionic gelation encapsulates 46.8 ± 5.0% Cip. The gel matrix can release Cip in a sustained manner, releasing 42.7 ± 0.2% in 2 h under simulated stomach pH conditions, and 83.3 ± 1.1% Cip release in 80 mM phosphate at pH = 7.40 (intestinal). The increase of sodium chloride from 50 to 200 mM implies a Cip release from 69.0 ± 1.5% to 95.1 ± 3.6% respectively in 2 h. Scanning electron microscopy revealed the cohesive effect of HM pectin over alginate molecules on the microsphere surface. Those results guarantee all Cip contained in the alginate/HM pectin microspheres could be released in an established kinetic profile along the gastrointestinal tract, avoiding the Cip undesirable side effects during absorption.

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Acknowledgments

The present work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Agencia Nacional de Promoción Científica y Técnica (ANPCyT) of Argentina. We want to thank Dr. Paul Dumas (SMIS beam line, Soleil Synchrotron Facility, France) for his kind support and expertise during the sample analysis. We thank CPKelco (Buenos Aires, Argentina) for the donation of pectin samples used in the present work.

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Authors and Affiliations

  1. Nanobiomaterials Laboratory, CINDEFI (CONICET, CCT La Plata)-School of Sciences, Universidad Nacional de La Plata, Calle 50 # 227, La Plata, 1900, Argentina

    Germán A. Islan & Guillermo R. Castro

  2. CINDECA (CONICET, CCT La Plata), Universidad Nacional de La Plata, Calle 47 # 257, La Plata, 1900, Argentina

    Ignacio Pérez de Verti & Sergio G. Marchetti

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  1. Germán A. Islan
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  2. Ignacio Pérez de Verti
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  3. Sergio G. Marchetti
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  4. Guillermo R. Castro
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Correspondence to Guillermo R. Castro.

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Islan, G.A., de Verti, I.P., Marchetti, S.G. et al. Studies of Ciprofloxacin Encapsulation on Alginate/Pectin Matrixes and Its Relationship with Biodisponibility. Appl Biochem Biotechnol 167, 1408–1420 (2012). https://doi.org/10.1007/s12010-012-9610-2

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  • DOI: https://doi.org/10.1007/s12010-012-9610-2

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