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Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system

  • Session 1A: Enzyme Catalysis And Engineering
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An Erratum to this article was published on 15 May 2008

Abstract

A sequential injection analysis system with two enzymatic microreactors for the determination of ethanol has been designed. Alcohol oxidase and horseradish peroxidase were separately immobilized on glass aminopropyl beads, and packed in 0.91-mL volume microreactors, working in line with the sequential injection analysis system. A stop flow of 120 s was selected for a linear ethanol range of 0.005–0.04 g/L ± 0.6% relative standard deviation with a throughput of seven analyses per hour. The system was applied to measure ethanol concentrations in samples of distilled and nondistilled alcoholic beverages, and of alcoholic fermentation with good performance and no significant difference compared with other analytical procedures (gas chromatography and high-performance liquid chromatography).

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Authors and Affiliations

  1. Escola de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitária, CEP: 21.949-900, Rio de Janeiro, Brasil

    Eliana M. Alhadeff, Andrea M. Salgado, Nei Pereira &  BelkisValdman

  2. Department d’Enginyeria Química, Escola Tècnica Superior d’Enginyeria, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Oriol Cos & Francisco Valero

Authors
  1. Eliana M. Alhadeff
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  2. Andrea M. Salgado
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  3. Oriol Cos
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  4. Nei Pereira
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  5. BelkisValdman
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  6. Francisco Valero
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Corresponding author

Correspondence to Eliana M. Alhadeff.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s12010-008-8201-8.

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Alhadeff, E.M., Salgado, A.M., Cos, O. et al. Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system. Appl Biochem Biotechnol 137, 17–25 (2007). https://doi.org/10.1007/s12010-007-9036-4

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  • DOI: https://doi.org/10.1007/s12010-007-9036-4

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