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Immobilization of hemoglobin on platinum nanoparticles-modified glassy carbon electrode for H2O2 sensing

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Wuhan University Journal of Natural Sciences

Abstract

This work described an amperometric hydrogen peroxide (H2O2) biosensor based on immobilization of hemoglobin (Hb) on a glassy carbon (GC) electrode modified by platinum nanoparticles, which was prepared by an in situ chemical reductive growth method. The electrochemical impedance measurements confirmed that the Hb was immobilized on the platinum nanoparticles-modified glassy carbon surface and has a synergistic effect with platinum nanoparticles in improving the catalytic reduction of H2O2. The Hb immobilized platinum nanoparticles-modified GC (Hb/Pt/GC) electrode displays an effective catalytic response to the reduction of H2O2. A linear dependence of the catalytic current versus H2O2 concentration was obtained in the range of 5.0×10−6 to 4.5×10−4 mol·L−1 with a detection limit (S/N=3) of 7.4×10−7 mol·L−1.

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Authors and Affiliations

  1. College of Resources and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, Hubei, China

    Gongming Qian

  2. School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, China

    Changzhu Yang, Jingdong Zhang & Wenhong Pu

Authors
  1. Gongming Qian
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  2. Changzhu Yang
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  3. Jingdong Zhang
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  4. Wenhong Pu
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Corresponding author

Correspondence to Changzhu Yang.

Additional information

Foundation item: Supported by Science Research Foundation of Huazhong University of Science and Technology (2006Z002A)

Biography: QIAN Gongming, male, Ph.D., research direction: environmental electrochemistry.

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Qian, G., Yang, C., Zhang, J. et al. Immobilization of hemoglobin on platinum nanoparticles-modified glassy carbon electrode for H2O2 sensing. Wuhan Univ. J. Nat. Sci. 15, 160–164 (2010). https://doi.org/10.1007/s11859-010-0214-9

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  • DOI: https://doi.org/10.1007/s11859-010-0214-9

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