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CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma

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Abstract

Background

CCK1 (cholecystokinin receptor 1) and protein lysine acetylation were associated with several cancers, respectively. However, whether they are involved in the alternation of gallbladder carcinoma is unknown.

Aims

This study investigated the characteristics of CCK1 and protein lysine acetylation in GBC-SD cells and carcinoma of gallbladder.

Methods

The expression and localization of CCK1 were detected by western blot analysis and indirect immunofluorescence. GBC-SD cells were treated with CCK-8 and CCK-8+CCK1 inhibitor. The protein lysine acetylation from cells, as well as tissues of gallbladder carcinoma, was examined by western blotting.

Results

CCK1 receptor was expressed and localized in the GBC-SD cells. The synthetic octapeptide of CCK (CCK-8) could accelerate the lysine acetylation of a subset of proteins in dose-dependent manners in GBC-SD cells. Further investigation demonstrated that the specific inhibitor (CR1409) of CCK1 receptor could attenuate the CCK8-induced increase of protein lysine acetylation. In addition, we revealed that the rise of CCK1 receptor expression is associated with the increase of protein lysine acetylation in tissues from carcinoma of gallbladder.

Conclusions

CCK might regulate protein lysine acetylation via CCK1 receptor.

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Acknowledgments

This work was supported by grants from Anhui University Natural Science Research Project (Grant No. KJ2015B077).

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Author notes

  1. Wei Wu and Bo Ouyang made equal contributions to this work.

Authors and Affiliations

  1. The First Affiliated Hospital of Bengbu Medical College, Chang Huai Road, Bengbu, Anhui, 233004, China

    W. Wu, Z. Lu, H. Liu, Y. Tan & P. Cui

  2. Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, 211100, China

    B. Ouyang

Authors
  1. W. Wu
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  2. B. Ouyang
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  3. Z. Lu
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  4. H. Liu
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  5. Y. Tan
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  6. P. Cui
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Corresponding authors

Correspondence to W. Wu or P. Cui.

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The authors declare that they have no competing interests.

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Wu, W., Ouyang, B., Lu, Z. et al. CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma. Ir J Med Sci 186, 883–888 (2017). https://doi.org/10.1007/s11845-017-1603-2

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  • DOI: https://doi.org/10.1007/s11845-017-1603-2

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