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Cobalt-Doped Brushite Cement: Preparation, Characterization, and In Vitro Interaction with Osteosarcoma Cells

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Abstract

Brushite cement (BrC) is being widely used in bone and dental tissue engineering application because of its significant biocompatibility, bioresorbability, and moldability. Here, we have reported the effects of cobalt (Co) and its concentration on physical and biological properties of BrC. Our results show that Co addition stabilizes the tricalcium phosphate structure and decreases the amount of BrC phase in the final product. The in vitro interaction of samples with osteosarcoma MG-63 cells proved the cytocompatibility of all compositions in both normoxic and hypoxic conditions. Although the cell viability increased under hypoxia, the change was insignificant compared with normoxic conditions. Our data show that Co addition reduced hypoxia inducible factor-1α and glioma-associated oncogene family zinc finger 2 expression in MG-63, suggesting Co may provide the benefit of reducing the effects of hypoxia on gene expression in the osteosarcoma cell line.

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Acknowledgements

The authors would like to acknowledge Mr. Gregg Westberg, Microelectronics Research and Development Lab (MRDL) manager, and Dr. Michael Haji-Sheikh, from the Department of Electrical Engineering at Northern Illinois University.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Northern Illinois University, 590 Garden Road, Dekalb, IL, 60115, USA

    Haley Cummings & Sahar Vahabzadeh

  2. Department of Biological Sciences, Northern Illinois University, 155 Castle Dr, Dekalb, IL, 60115, USA

    Weiguo Han & Sherine F. Elsawa

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  1. Haley Cummings
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  2. Weiguo Han
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  3. Sahar Vahabzadeh
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  4. Sherine F. Elsawa
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Correspondence to Sahar Vahabzadeh.

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Cummings, H., Han, W., Vahabzadeh, S. et al. Cobalt-Doped Brushite Cement: Preparation, Characterization, and In Vitro Interaction with Osteosarcoma Cells. JOM 69, 1348–1353 (2017). https://doi.org/10.1007/s11837-017-2376-9

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  • DOI: https://doi.org/10.1007/s11837-017-2376-9

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