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Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance

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Abstract

Groundnut (Arachis hypogaea L.) is an important oilseed crop grown in semi-arid tropics where it experiences moisture stress at different stages of growth resulting in reduced growth and productivity. In this study, we report that the stress tolerance of groundnut can be improved by overexpression of stress-specific transcription factor through transgenic approach. In silico electronic-northern analysis of AtNAC2 showed increased expression under different abiotic stresses. The transcript levels of a homolog of AtNAC2 gene were upregulated under different drought regimes in groundnut. Groundnut transgenics overexpressing AtNAC2 showed enhanced tolerance to drought and salinity with improved yield under water-limited conditions. The study demonstrates that AtNAC2 is a potential candidate gene to improve stress tolerance by transgenic approach.

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References

  • Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M (1997) Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell 9:841–857

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Akcay UC, Ercan O, Kavas M, Yildiz L, Yilmaz OA, Yucel M (2010) Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings. Plant Growth Regul 61:21–28

    Article  Google Scholar 

  • Anami S, De Block M, Machuka J, Van LM (2009) Molecular improvement of tropical maize for drought stress tolerance in sub-Saharan Africa. Crit Rev Plant Sci 28:16–35

    Article  CAS  Google Scholar 

  • Babitha KC, Ramu SV, Pruthvi V, Mahesh P, Nataraja NK, Udayakumar M (2013) Co-expression of AtbHLH17 and AtWRKY28 confers resistance to abiotic stress in Arabidopsis. Transgenic Res 22:327–341

    Article  CAS  PubMed  Google Scholar 

  • Barrs HO, Weatherly PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413–428

    Google Scholar 

  • Bhatnagar MP, Vadez V, Sharma KK (2008) Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep 27:411–424

    Article  Google Scholar 

  • Datta SK, Patel H, Berry D (1989) Extraction and purification of RNA from crop plants. J Exp Bot 165:1252

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Fang Y, You J, Xie K, Xie W, Xiong L (2008) Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Genet Genomics 28:547–563

    Article  Google Scholar 

  • Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran LS, Yamaguchi-Shinozaki K, Shinozaki K (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J 39:863–876

    Article  CAS  PubMed  Google Scholar 

  • Govind G, Harshavardhan VT, Patricia JK, Dhanalakshmi R, Senthil-Kumar M, Sreenivasulu N, Udayakumar M (2009) Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol Genet Genomics 281:591–605

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • He X, Mu R, Cao W, Zhang Z, Zhang J, Chen S (2005) AtNAC2, a transcription factor downstream of ethylene and auxin signalling pathways, is involved in salt stress response and lateral root development. Plant J 44:903–916

    Article  CAS  PubMed  Google Scholar 

  • Hoekstra PA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccations tolerance. Trends Plant Sci 6:431–438

    Article  CAS  PubMed  Google Scholar 

  • Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA 103:12987–12992

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hu H, You J, Fang Y, Zhu X, Qi Z, Xiong L (2008) Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Mol Biol 67:169–181

    Article  CAS  PubMed  Google Scholar 

  • Jenson MK, Rung JH, Gregerson PL, Gjetting T, Fugslang AT, Hansen M (2007) The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis. Plant Mol Biol 65:141–152

    Google Scholar 

  • Jeong JS, Kim YS, Baek KH, Jung H, Ha SH, Do Choi Y, Kim M, Reuzeau C, Kim JK (2010) Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiol 153:185–197

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Karaba A, Dixit S, Greco R, Aharoni A, Trijatmiko KR, Marsch-Martine N, Krishnan A, Nataraja KN, Udayakumar M, Pereira A (2007) Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene. Proc Natl Acad Sci USA 104:15270–15275

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Levitt J (1980) Responses of plants to environmental stresses: water, radiation, salt and other stresses, vol 2. Academic, New York, pp 3–211

    Google Scholar 

  • Liu X, Hong L, Li XY, Yao Y, Hu B, Li L (2011) Improved drought and salt tolerance in transgenic Arabidopsis overexpressing a NAC transcriptional factor from Arachis hypogaea. Biosci Biotechnol Biochem 75:443–450

    Article  CAS  PubMed  Google Scholar 

  • Mao X, Zhang H, Qian X, Li A, Zhao G, Jing R (2012) TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. J Exp Bot 63:2933–2946

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mitsuda N, Ohme-Takagi M (2008) NAC transcription factors NST1 and NST3 regulate pod shattering in a partially redundant manner by promoting secondary wall formation after the establishment of tissue identity. Plant J 56:768–778

    Article  CAS  PubMed  Google Scholar 

  • Mukhopadhyay A, Vij S, Tyagi AK (2004) Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Natl Acad Sci USA 101:6309–6314

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Nakashima K, Takasaki H, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2012) NAC transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819:97–103

    Article  CAS  PubMed  Google Scholar 

  • Nambara E, Marion-Poll A (2005) Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol 56:165–185

    Article  CAS  PubMed  Google Scholar 

  • Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res 10:239–242

    Article  CAS  PubMed  Google Scholar 

  • Puckette MC, Weng H, Mahalingam R (2007) Physiological and biochemical responses to acute ozone-induced oxidative stress in Medicago truncatula. Plant Physiol Biochem 45:70–79

    Article  CAS  PubMed  Google Scholar 

  • Puranik S, Sahu PP, Srivastava SP, Prasad M (2012) NAC proteins: regulation and role in stress tolerance. Trends Plant Sci 17:1360–1385

    Article  Google Scholar 

  • Ramegowda V, Senthil-Kumar M, Nataraja NK, Reddy KM, Mysore SK, Udayakumar M (2012) Expression of a finger millet transcription factor, EcNAC1, in tobacco confers abiotic stress-tolerance. PLoS ONE 7(7):e40397

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ramu SV, Rohini S, Keshavareddy G, Gowri Neelima M, Shanmugam NB, Kumar ARV, Sarangi SK, Ananda Kumar P, Udayakumar M (2012) Expression of a synthetic cry1AcF gene in transgenic Pigeon pea confers resistance to Helicoverpa armigera. J Appl Entomol 136(9):675–687

    Article  CAS  Google Scholar 

  • Riechmann JL, Reuber L, Jiang Z, Keddie L, Adam O, Pineda O, Ratcliffe R, Samaha R, Creelman R, Pilgrim P, Broun J, Zhang Z, Ghandehari B, Sherman K, Yu L (2002) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110

    Article  Google Scholar 

  • Rohini VK, Sankara Rao K (2000) Transformation of peanut (Arachis hypogaea L.): a non-tissue culture based approach for generating transgenic plants. Plant Sci 150:41–49

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory Press, Plain view

    Google Scholar 

  • Shinozaki K, Dennis ES (2003) Cell signalling and gene regulation global analyses of signal transduction and gene expression profiles. Curr Opin Plant Biol 6:405–409

    Article  CAS  PubMed  Google Scholar 

  • Tran LS, Nakashima K, Sakuma Y, Simpson D, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xia N, Zhang G, Liu X, Deng L, Cai G, Zhang Y, Wang X, Zhao J, Huang L, Kang Z (2010a) Characterization of a novel wheat NAC transcription factor gene involved in defense response against stripe rust pathogen infection and abiotic stresses. Mol Biol Rep 37:3703–3712

    Article  CAS  PubMed  Google Scholar 

  • Xia N, Zhang G, Sun YF, Zhu L, Xu LS, Chen XM, Liu B, Yu YT, Wang XJ, Huang LL, Kang ZS (2010b) TaNAC8, a novel NAC transcription factor gene in wheat, responds to stripe rust pathogen infection and abiotic stresses. Physiol Mol Plant Pathol 74:394–402

    Article  CAS  Google Scholar 

  • Xie Q, Frugis G, Colgan D, Chua NH (2000) Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development. Genes Dev 14:3024–3036

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yanhui C, Xiaoyuan Y, Kun H, Meihua L, Jigang L, Zhaofeng G, Zhiqiang L, Yunfei Z, Xiaoxiao W, Xiaoming Q, Yunping S, Li Z, Xiaohui D, Jingchu L, Xing-Wang D, Zhangliang C, Hongya G, Li-Jia Q (2006) The MYB transcription factor superfamily of Arabidopsis: expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol Biol 60:107–124

    Article  PubMed  Google Scholar 

  • Yao D, Zhang X, Zhao X, Liu C, Wang C, Zhang Z, Zhang C, Wei Q, Wang Q, Yan H, Li F, Su Z (2011) Transcriptase analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.). Genomics 98:47–55

    CAS  PubMed  Google Scholar 

  • Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61:672–685

    Article  CAS  PubMed  Google Scholar 

  • Zheng X, Chen B, Lu G, Han B (2009) Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochem Biophys Res Commun 379:985–989

    Article  CAS  PubMed  Google Scholar 

  • Zhu J (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

Authors acknowledge the financial support from Department of Biotechnology, Centre of Excellence programme support (BT/01/COE/05/03), Indian Council of Agricultural Research—Niche area of Excellence programme (F. No. 10-(6)/2005 EP&D) and Department of Science and Technology—Fund for Improvement of Science and Technology (SR/FST/LSI-051/2002).

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  1. Rohini Sreevathsa

    Present address: NRCBP, New Delhi, India

Authors and Affiliations

  1. Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065, India

    Mahesh Patil, S. V. Ramu, P. Jathish, Rohini Sreevathsa, P. Chandrashekar Reddy, T. G. Prasad & M. Udayakumar

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  1. Mahesh Patil
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Correspondence to T. G. Prasad.

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Patil, M., Ramu, S.V., Jathish, P. et al. Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance. Plant Biotechnol Rep 8, 161–169 (2014). https://doi.org/10.1007/s11816-013-0305-0

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