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Characterization and fine mapping of a shortened uppermost internode mutant in rice

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Abstract

Internode elongation is an important agronomic trait in rice that is associated with lodging, yield, flooding adaptation, and hybrid seed production. We identified a novel rice mutant line showing a shortened uppermost internode among the rice Ac/Ds insertional mutant population and named it shortened uppermost internode 4 (sui4). Interestingly, T1 plants of this line segregated into three phenotypes: extremely shortened uppermost internode-type, normal wild-type, and intermediate-type. The phenotypes of F1 plants and F2 plants from the cross of sui4 with its original variety, Dongjin, indicated that the SUI4 gene shows incomplete dominance or semidominance. Because the Ds genotypes did not co-segregate with the sui4 phenotypes, we performed mapping of this gene with 273 F2 plants from a cross between sui4 and Milyang23. Primary mapping revealed that the SUI4 locus was located between the S07012 and S07015 markers on rice chromosome 7. Further fine mapping with 36 F3 lines derived from F2 plants that have recombination in this region narrowed down the location of SUI4 to the 1.1-Mbp interval of RM1253–S07015.

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Acknowledgments

This research was supported by grants from the National Academy of Agricultural Science (NAAS), project code PJ006817, Republic of Korea and the Next Generation Biogreen 21 Program, Rural Development Administration, project code PJ008091.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, National Academy of Agricultural Science, Suwon, 441-707, Republic of Korea

    Hyeonso Ji, Hakbum Kim, Ung-Han Yoon, Tae-Ho Kim & Gang-Seob Lee

  2. Planning and Coordination Division, National Academy of Agricultural Science, Suwon, 441-707, Republic of Korea

    Doh-Won Yun

  3. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Moo-Young Eun

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  1. Hyeonso Ji
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Correspondence to Gang-Seob Lee.

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Ji, H., Kim, H., Yun, DW. et al. Characterization and fine mapping of a shortened uppermost internode mutant in rice. Plant Biotechnol Rep 8, 125–134 (2014). https://doi.org/10.1007/s11816-013-0280-5

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