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Potential industrial application of Actinobacillus succinogenes NJ113 for pyruvic acid production by microaerobic fermentation

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Abstract

Actinobacillus succinogenes NJ113 is capable of microaerobic fermentation, which offers the possibility of a novel type of pyruvic acid production. A dissolved oxygen environment with stirring at 300 rpm was a key factor in the fermentative production of a maximum concentration of pyruvic acid. Potassium carbonate (K2CO3) was found to have a role in promoting pyruvic acid production, influencing the concentration of pyruvic acid and production of the by-product succinic acid. The final titer of pyruvic acid production was 36.8±0.1 g L−1 with an overall yield of 0.639±0.056 g g−1 glucose and 3.12±0.03mmol g−1 dry cell weight h−1.

Significance and impact of the study

This study is the first to illustrate the advantage of using Actinobacillus succinogenes NJ113 with no genetic modification under microaerobic conditions for the production of pyruvic acid.

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References

  1. G. C. Paul and C.R. Thomas, Adv. Biochem. Eng./Biotechnol., 60, 1 (1998).

    CAS  Google Scholar 

  2. J. B. McKinlay, C. Vieille and J. G. Zeikus, Appl. Microbiol. Biotechnol., 76, 727 (2007).

    Article  CAS  Google Scholar 

  3. Y. Li, J. Chen and S. Y. Lun, Appl. Microbiol. Biotechnol., 57, 451 (2001).

    Article  CAS  Google Scholar 

  4. Q. Wang, P. He, D. Lu, A. Shen and N. Jiang, Lett. Appl. Microbiol., 35, 338 (2002).

    Article  CAS  Google Scholar 

  5. P. Xu, J. H. Qiu, C. Gao and C.Q. Ma, J. Bioscience Bioengineering, 105, 169 (2008).

    Article  CAS  Google Scholar 

  6. V.F. Wendisch, M. Bott and B.J. Eikmanns, Current Opinion Microbiol., 9, 268 (2006).

    Article  CAS  Google Scholar 

  7. S. Wieschalka, B. Blombach and B. J. Eikmanns, Appl. Microbiol. Biotechnol., 94, 449 (2012).

    Article  CAS  Google Scholar 

  8. J. B. McKinlay, M. Laivenieks, B.D. Schindler, A. A. McKinlay, S. Siddaramappa, J.F. Challacombe, S.R. Lowry, A. Clum, A.L. Lapidus, K. B. Burkhart, V. Harkins and C. Vieille, Bmc Genomics, 11, 16 (2010).

    Article  Google Scholar 

  9. M.V. Guettler, D. Rumler and M. K. Jain, Int. J. Systematic Bacteriology, 49, 207 (1999).

    Article  CAS  Google Scholar 

  10. Q. Li, D. Wang, Z. Song, W. Zhou, Y. Wu, J. Xing and Z. Su, Bioresour. Technol., 101, 7665 (2010).

    Article  CAS  Google Scholar 

  11. Y.-l. Xi, K.-q. Chen, R. Xu, J.-h. Zhang, X.-f. Bai, M Jiang, P. Wei and J.-y. Chen, Biochem. Eng. J., 69, 87 (2012).

    Article  CAS  Google Scholar 

  12. M.R. Leonardo, Y. Dailly and D. P. Clark, J. Bacteriol., 178, 6013 (1996).

    CAS  Google Scholar 

  13. I. Martinez, G. N. Bennett and K.Y. San, Metabolic Engineering, 12, 499 (2010).

    Article  CAS  Google Scholar 

  14. C. Seo, H.W. Lee, A. Suresh, J.W. Yang, J. K. Jung and Y. C. Kim, Korean J. Chem. Eng., 31, 1433 (2014).

    Article  CAS  Google Scholar 

  15. G. Sawers, Current Opinion Microbiol., 2, 181 (1999).

    Article  CAS  Google Scholar 

  16. J. B. McKinlay, Y. Shachar-Hill, J. G. Zeikus and C. Vieille, Metabolic Engineering, 9, 177 (2007).

    Article  CAS  Google Scholar 

  17. J. Wang, J. F. Zhu, G.N. Bennett and K.Y. San, Metabolic Engineering, 13, 328 (2011).

    Article  CAS  Google Scholar 

  18. S. Alexeeva, B. de Kort, G. Sawers, K. J. Hellingwerf and M. J.T. de Mattos, J. Bacteriol., 182, 4934 (2000).

    Article  CAS  Google Scholar 

  19. A. J.A. van Maris, J.M.A. Geertman, A. Vermeulen, M.K. Groothuizen, A.A. Winkler, M.D.W. Piper, J.P. van Dijken and J.T. Pronk, Appl. Environ. Microbiol., 70, 159 (2004).

    Article  Google Scholar 

  20. B. Zelic, S. Gostovic, K. Vuorilehto, B. Vasic-Racki and R. Takors, Biotechnology and Bioengineering, 85, 638 (2004).

    Article  CAS  Google Scholar 

  21. Y. Izumi, Y. Matsumura, Y. Tani and H. Yamada, Agricultural and Biological Chemistry, 46, 2673 (1982).

    CAS  Google Scholar 

  22. H. Yanase, N. Mori, M. Masuda, K. Kita, M. Shimao and N. Kato, J. Fermentation Bioengineering, 73, 287 (1992).

    Article  CAS  Google Scholar 

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Correspondence to Kequan Chen.

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Wang, Z., Xiao, W., Zhang, A. et al. Potential industrial application of Actinobacillus succinogenes NJ113 for pyruvic acid production by microaerobic fermentation. Korean J. Chem. Eng. 33, 2908–2914 (2016). https://doi.org/10.1007/s11814-016-0168-5

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  • DOI: https://doi.org/10.1007/s11814-016-0168-5

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