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Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot (Scophthalmus maximus)

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Abstract

In this study, juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda (EIBAV1) and reared at two different densities, low density (LD), (5.25±0.02)kgm−2, as control group and high density (HD), (20.53±0.05) kg m−2, as experimental group. Only density was considered as the variable. Five weeks after vaccination, the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology. A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N50 size of 2295 bp. A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes (DEGs). Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway, cytosolic DNA-sensing pathway and platelet activation in the host immune responses. The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs. It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.

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Acknowledgements

This work was supported by the National Key R&D Program of China (No. 2017YFB0404001), the Central Public-Interest Scientific Institution Basal Research Fund, CAFS (No. 2017HYZD04), the Qingdao Shinan District Science and Technology Bureau (No. 2016-3-006) and the Modern Agriculture Industry System Construction Special Funds (No. CARS-47-G24).

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  1. Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Huanhuan Huo, Xiaoqiang Gao, Fan Fei, Fei Qin, Bin Huang & Baoliang Liu

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Baoliang Liu

  3. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China

    Huanhuan Huo

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Huo, H., Gao, X., Fei, F. et al. Transcriptomic Profiling of the Immune Response to Crowding Stress in Juvenile Turbot (Scophthalmus maximus). J. Ocean Univ. China 19, 911–922 (2020). https://doi.org/10.1007/s11802-020-4242-6

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