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Distribution and elimination of Norfloxacin in Fenneropenaeus chinensis larvae

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Abstract

This study examined the distribution and elimination of Norfloxacin (NFLX) in Fenneropenaeus chinensis ovary and egg and newly hatched larvae. Mature parental shrimp were exposed to 4 or 10 mg L−1 NFLX for 2 or 5 d. Ovary and eggs of the shrimp were sampled after spawning in order to detect NFLX residue using high-performance liquid chromatography (HPLC). Results showed that NFLX residue accumulated in F. chinensis eggs after the parental exposure, with the highest residue detected in ovary. To examine the fate of NFLX residue in larvae, we further determined the concentration of NFLX residue in F. chinensis eggs and larvae at 4 different developmental stages after 24-h exposure. From the newly metamorphosed larvae (0 h post-metamorphosis, h.p.m), samples were taken at different time intervals to 72 h.p.m. HPLC assay showed that the concentrations of NFLX residue in zoea exposed to 4 and 10 mg L−1 NFLX were the highest at 1.5 h, i.e., 0.332 and 0.454 μg g−1, respectively. At the two NFLX exposure levels, the elimination time of half NFLX (half life) in nauplius was 45.36 and 49.85 h, respectively, followed by that in zoea (31.68 and 33.13 h), mysis larvae (42.24 and 47.28 h) and postlarvae (24.48 and 30.96 h). Both NFLX exposure levels had a germicidal effect. The distribution and elimination of NFLX residue in F. chinensis tissue, eggs and larvae correlated well with the drug exposure level. The disappearance of NFLX residue coincided with the larval growth, and the half-life of NFLX decreased with the larval development.

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Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao, 266003, P. R. China

    Ming Sun

  2. Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture of China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Ming Sun, Jian Li, Fazhen Zhao, Jitao Li & Zhiqiang Chang

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  1. Ming Sun
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  2. Jian Li
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  3. Fazhen Zhao
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  4. Jitao Li
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  5. Zhiqiang Chang
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Correspondence to Jian Li.

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Sun, M., Li, J., Zhao, F. et al. Distribution and elimination of Norfloxacin in Fenneropenaeus chinensis larvae. J. Ocean Univ. China 12, 397–402 (2013). https://doi.org/10.1007/s11802-013-2179-8

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  • DOI: https://doi.org/10.1007/s11802-013-2179-8

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