Abstract
In order to solve the citrus peel resource waste problem and minimize the drawbacks of chemical extraction of pectin, a protopectinase-overproducing strain CD-01 for pectin production was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence (FJ184995) as that of Aspergillus niger (ATCC 64028). It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L9(34) orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin is as follows: time 36 h, temperature 35 °C, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus.
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Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China
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Xia, Jl., Meng, H., Wang, Rm. et al. Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production. J. Cent. South Univ. Technol. 16, 53–60 (2009). https://doi.org/10.1007/s11771-009-0009-3
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DOI: https://doi.org/10.1007/s11771-009-0009-3