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Transcriptome profiles underlying the spermidine-elevated resistance to high temperature in the radish Raphanus sativus

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Abstract

Radish is a common crop and vulnerable to high temperature. Pretreatment with spermidine could effectively elevate the resistance of radish to high temperature. Up to date, the underlying mechanisms remain unknown. In the present study, radish seedlings were pretreated with spermidine and then exposed to high temperature. The leaves were collected for transcriptome sequencing and the results were compared with samples only exposed to high temperature and samples consistently cultured at normal temperature. The differentially expressed genes were identified and validated using real-time quantitative PCR. Finally, the results revealed that pretreatment with spermidine significantly up-regulated the transcription level of anti-oxidation enzymes, heat-shock proteins, and proteins involved in the photosynthetic process, in comparison with treatments with high temperature and room temperature, suggesting that pretreatment with spermidine increased heat tolerance in radish probably through minimizing the harms caused by peroxidation, accelerating the corrections or degradation of misfolded proteins, and promoting photosynthesis. These findings preliminarily uncovered the molecular mechanisms underlying the spermidine-promoted heat resistance in radish.

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Acknowledgements

We thank Miss Mei Lin from the Shenzhen GenProMetab Biotechnology Co., Ltd. for the assistance in real-time qPCR tests and the Shenzhen Nobel Science and Technology Service Co., Ltd. for the comments and corrections of English. This work was supported by Projects in Jiangsu Agricultural Science and Technology Innovation Fund [no. CX(13)5082].

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  1. Yi Mei and Jun Guo contribute equally to this work.

Authors and Affiliations

  1. Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng, 224002, Jiangsu, People’s Republic of China

    Yi Mei, Jun Guo, Yun-Fen Liu, Yan-Xia Zu, Yong-Cheng Wu, Jia-Qiu Zheng & Wei-Wei Wang

  2. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Science, Nanjing, 210014, Jiangsu, People’s Republic of China

    Xiao-Jun Su

  3. Heyuan Entry-exit Inspection and Quarantine Bureau, Heyuan, 517000, Guangdong, People’s Republic of China

    Ning Ding

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  1. Yi Mei
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  2. Jun Guo
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  4. Yun-Fen Liu
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Correspondence to Jun Guo.

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Communicated by M. Stobiecki.

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Mei, Y., Guo, J., Ding, N. et al. Transcriptome profiles underlying the spermidine-elevated resistance to high temperature in the radish Raphanus sativus. Acta Physiol Plant 40, 109 (2018). https://doi.org/10.1007/s11738-018-2679-7

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  • DOI: https://doi.org/10.1007/s11738-018-2679-7

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