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Differential accumulation of proteins in leaves and roots associated with heat tolerance in two Kentucky bluegrass genotypes differing in heat tolerance

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Abstract

To understand the mechanisms of heat stress responses in perennial grasses, differential proteins in leaves and roots of two genotypes of Kentucky bluegrass (Poa pratensis), including heat-tolerant ‘Midnight’ and heat-sensitive ‘Brilliant’, were analyzed with two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). Plants were exposed to heat stress for 28 days in growth chambers. Under 7–28 days of heat stress, leaf photochemical efficiency declined significantly while electrolyte leakage increased in leaves and roots, and to a lesser extent for heat-tolerant ‘Midnight’ than for heat-sensitive ‘Brilliant’. Compared with leaves, cell membrane damage due to heat stress was more severe in roots. The 2-DE and MS analysis identified 37 heat-responsive proteins in leaves, 28 heat-responsive proteins in roots; 14 proteins in leaves and 9 proteins in roots exhibited differential expression between the two genotypes. The results indicate that proteins involved in metabolism and energy in leaves and those in antioxidant defense in roots are associated with heat tolerance in Kentucky bluegrass. The differential accumulation of these proteins might be the reason for different heat tolerance in two Kentucky bluegrass genotypes in aerial and underground parts.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

MS:

Mass spectrometry

Hsp:

Heat-shock proteins

EL:

Electrolyte leakage

Fv/Fm:

The ratio of variable to maximum fluorescence

DTT:

Dithiothreitol

CHAPS:

3-[(3-Cholamidopropyl) dimethylammoniol]-1-propane-sulfonate

IPG:

Immobilized pH gradient

IEF:

Isoelectric focusing

MALDI-TOF/TOF:

Matrix-assisted laser desorption/ionization time-of-flight/time-of-flight

ESI-MS:

Electrospray ionization mass spectrometry

CS:

Cysteine synthase

TCA:

Tri-carboxylic-acid

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

Cyt 450:

Cytochrome P450

POD:

Peroxidase

DHAR:

Dehydroascorbate reductase

TPI:

Triosephosphate isomerase

3-PGK:

3-Phosphoglycerate kinase

GSH:

Glutathione

LGL:

Lactoylglutathionelyase

MG:

Methylglyoxal

ADK:

Adenosine kinase

ROS:

Reactive oxygen species

GLPs:

Germin-like proteins

NAC:

Nascent polypeptide-associated complex

P:

Phosphorus

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Acknowledgments

The authors wish to thank National Natural Science Foundation of China (Grant No. 30900992) for funding support.

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  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Yuting Zhang & Hongmei Du

  2. Shanghai Urban Forest Research Station, State Forestry Administration, Shanghai, 200240, China

    Hongmei Du

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  1. Yuting Zhang
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Correspondence to Hongmei Du.

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Communicated by H. Peng.

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Zhang, Y., Du, H. Differential accumulation of proteins in leaves and roots associated with heat tolerance in two Kentucky bluegrass genotypes differing in heat tolerance. Acta Physiol Plant 38, 213 (2016). https://doi.org/10.1007/s11738-016-2232-5

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  • DOI: https://doi.org/10.1007/s11738-016-2232-5

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