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Regeneration from chrysanthemum flowers: a review

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Abstract

The use of flower tissue for the in vitro propagation of chrysanthemum (Chrysanthemum × grandiflorum (Ramat.) Kitam.), an ornamental plant, provides a specific and precious explant type for organogenesis (regeneration of adventitious shoots/roots) or somatic embryogenesis. This is of great importance for the breeding of this species. In vitro culture of flower tissue can lead to the separation of chimera components to obtain stable mutants. Moreover, regenerants derived from ray (ligulate) or disc (tubular) florets may present somaclonal variation. The ability to then regenerate such plant material can result in the production of plants with novel flower colour, modified architecture or other positive attributes, even more so after the application of physical mutagens such as gamma radiation. Although in vitro flowering is a rare phenomenon in chrysanthemum, the notion that it could be possible would allow for the formation of flower material that could serve as in vitro-disinfected material without the need to introduce explants, and possible contamination, from the ex vivo milieu. Regeneration protocols from flower tissue would allow for breeding using such tissues to be advanced while the process of in vitro flowering could also be better understood. This review provides an up-to-date understanding of what is known in this field of research.

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Acknowledgments

The authors thank Dr. Haenghoon Kim (Sunchon National University, South Korea) and the anonymous reviewer(s) for providing useful comments on the manuscript. The authors thank Dr. D. J. Nicholas Hind, Head of Compositae Research, Royal Botanic Gardens, Kew, UK, for fruitful discussion on and clarification of the nomenclature and taxonomy of garden chrysanthemums.

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The authors declare no conflicts of interest, financial or other.

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Authors and Affiliations

  1. Ikenobe 3011-2, Miki-cho Post Office, P. O. Box 7, Kagawa, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, UTP University of Science and Technology, Bernardyńska 6, 85-029, Bydgoszcz, Poland

    Justyna Lema-Rumińska & Alicja Tymoszuk

  3. Department of Genetics, Plant Breeding, and Biotechnology, West-Pomeranian University of Technology, Janosika 8, 71-424, Szczecin, Poland

    Danuta Kulpa

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  1. Jaime A. Teixeira da Silva
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  2. Justyna Lema-Rumińska
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  3. Alicja Tymoszuk
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Correspondence to Jaime A. Teixeira da Silva, Justyna Lema-Rumińska, Alicja Tymoszuk or Danuta Kulpa.

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Communicated by A. K. Kononowicz.

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Teixeira da Silva, J.A., Lema-Rumińska, J., Tymoszuk, A. et al. Regeneration from chrysanthemum flowers: a review. Acta Physiol Plant 37, 36 (2015). https://doi.org/10.1007/s11738-015-1773-3

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