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The natural alkaloid sanguinarine promotes the expression of heat shock protein genes in Arabidopsis

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Abstract

Small-molecule heat shock response inducers are known to enhance heat tolerance in plants. In this paper, we report that a plant alkaloid enhances the heat tolerance of Arabidopsis. We investigated 12 commercially available alkaloids to determine whether they enhance the heat tolerance of Arabidopsis seedlings using an in vitro assay system with geldanamycin, which is a known heat shock response inducer, as a positive control. Accordingly we found that the isoquinoline alkaloid sanguinarine can enhance heat tolerance in Arabidopsis. No such effect was shown for the other 11 alkaloids. The sanguinarine treatment increased the expression of heat shock protein genes such as HSP17.6C-CI, HSP70, and HSP90.1, which were up-regulated by geldanamycin. Treatments with other isoquinoline alkaloids (berberine and papaverine), which showed few heat tolerance-enhancing effects, did not promote the expression of the heat shock protein genes. These results suggest that sanguinarine influenced the heat tolerance of Arabidopsis by enhancing the expression of heat shock protein genes.

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Acknowledgments

This study was supported in part by a Grant-in-Aid (No. 21658025) for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, a grant from the Kurita Water and Environment Foundation, a grant for Environmental Research Projects from the Sumitomo Foundation, and the Incubate and Promote New Industry Organization.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, 422-8529, Japan

    Masakazu Hara & Ikuya Kurita

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  1. Masakazu Hara
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  2. Ikuya Kurita
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Correspondence to Masakazu Hara.

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Communicated by M. Hajduch.

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Hara, M., Kurita, I. The natural alkaloid sanguinarine promotes the expression of heat shock protein genes in Arabidopsis . Acta Physiol Plant 36, 3337–3343 (2014). https://doi.org/10.1007/s11738-014-1681-y

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  • DOI: https://doi.org/10.1007/s11738-014-1681-y

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