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Magnetopriming circumvents the effect of salinity stress on germination in chickpea seeds

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Abstract

Chickpea seeds of Pusa 1053 (Mediterranean) and Pusa 256 (native) were magnetoprimed with 100 mT static magnetic field for 1 h to evaluate the effect of magnetopriming on germination of seeds under saline conditions. Enhanced rate of germination and seedling growth parameters (root and shoot length, and vigour indices) under different salinity levels indicated that magnetopriming was more effective in alleviating salinity stress at early seedling stage in Pusa 1053 as compared to Pusa 256. Dynamics of seed water absorption in magnetoprimed seeds showed increased water uptake in Pusa 1053 under non-saline as compared to saline conditions. This could have resulted in faster hydration of enzymes in primed seeds leading to higher rate of germination. Total amylase, protease and dehydrogenase activities were higher in primed seeds as compared to unprimed seeds under both non-saline and saline conditions. Production of superoxide radicals was enhanced in germinating seeds of both the genotypes under salinity irrespective of priming. Increased levels of hydrogen peroxide in germinating magnetoprimed seeds, under both the growing conditions, suggested its role in promotion of germination. Our results showed that magnetopriming of dry seeds of chickpea can be effectively used as a pre-sowing treatment for mitigating adverse effects of salinity at seed germination and early seedling growth.

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Acknowledgments

ST thanks ICAR for Junior Research Fellowship to conduct this work. The authors also thank Head, Division of Plant Physiology for providing the facilities to conduct the experiments. The work was funded by I.A.R.I.

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Sini Thomas, Anjali Anand & Viswanthan Chinnusamy

  2. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India

    Anil Dahuja

  3. Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Sudipta Basu

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  1. Sini Thomas
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  2. Anjali Anand
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Correspondence to Anjali Anand.

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Communicated by M. Horbowicz.

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Thomas, S., Anand, A., Chinnusamy, V. et al. Magnetopriming circumvents the effect of salinity stress on germination in chickpea seeds. Acta Physiol Plant 35, 3401–3411 (2013). https://doi.org/10.1007/s11738-013-1375-x

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