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Changes in phenolic composition and antioxidant activities of the edible halophyte Crithmum maritimum L. with physiological stage and extraction method

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Abstract

Most antioxidant phytochemicals isolated from higher plants are polyphenols exhibiting different biological activities. Previous studies have shown that plant phenolic contents and antioxidant activities depend on several factors, mainly environmental conditions, and post-harvesting procedures. The present study aims to assess the influence of these interfering factors on phenolic composition and antioxidant activities (DPPH test, reducing power) of Crithmum maritimum L., a promising food halophyte which attracts an increasing interest in recent years. Results showed that the summer plants (reproductive stage) were richer on phenolic compounds than spring ones (vegetative stage), and consequently exhibited higher antioxidant activities. The comparison of two extraction methods showed that maceration extracts contained greatest amounts of phenolic compounds, while soxhlet ones exhibited higher antiradical and reducing power capacities. Besides, the analysis of variance disclosed that the effect of extraction method (E) was the predominant contributor to these antioxidant properties than the physiological development stage (D) and their interaction (E × D). Chromatographic characterization of C. maritimum phenolic composition using the RP-HPLC revealed the presence of phenolic acids and flavonoids with epigallocatechin as major component. Almost all of the identified compounds were described in the literature as interesting bioactive natural substances that may be used in several fields, such as nutraceuticals, cosmetics and agro-food industry.

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Abbreviations

AlCl3·6H2O:

Aluminum chloride hexahydrate solution

AscA:

Ascorbic acid

CE:

Catechin equivalents

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

EC50 (μg/ml):

Effective concentration at which the absorbance was 0.5

FeCl3 :

Iron(III)chloride anhydrous

GAE:

Gallic acid equivalents

HCl:

Hydrochloric acid

IC50 (μg/ml):

Inhibiting concentration of 50% of the synthetic radical DPPH

K3Fe (CN)6 :

Potassium ferricyanide (III)

mg CE g−1 DW:

Milligram catechin equivalent per gram dry weight

mg GAE g−1 DW:

Milligram gallic acid equivalent per gram dry weight

Na2CO3 :

Sodium carbonate anhydrous

NaNO2 :

Sodium nitrite

NaOH:

Sodium hydroxide

RP-HPLC:

Reverse phase high performance liquid chromatography

TCA:

Trichloroacetic acid

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Acknowledgments

This work was supported by the Tunisian Ministry of Higher Education and Scientific Research (LR10CBBC02).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie à la Technopole de Borj Cédria (CBBC), BP 901, 2050, Hammam-lif, Tunisia

    Ines Jallali, Wided Megdiche, Samia Oueslati, Abderrazek Smaoui, Chedly Abdelly & Riadh Ksouri

  2. Unité des Plantes Aromatiques et Médicinales, Centre de Biotechnologie à la Technopole de Borj Cédria (CBBC), BP 901, 2050, Hammam-lif, Tunisia

    Baya M’Hamdi

Authors
  1. Ines Jallali
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  2. Wided Megdiche
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  3. Baya M’Hamdi
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  4. Samia Oueslati
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  5. Abderrazek Smaoui
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  6. Chedly Abdelly
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  7. Riadh Ksouri
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Correspondence to Ines Jallali.

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Communicated by L. Bavaresco.

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Jallali, I., Megdiche, W., M’Hamdi, B. et al. Changes in phenolic composition and antioxidant activities of the edible halophyte Crithmum maritimum L. with physiological stage and extraction method. Acta Physiol Plant 34, 1451–1459 (2012). https://doi.org/10.1007/s11738-012-0943-9

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  • DOI: https://doi.org/10.1007/s11738-012-0943-9

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