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Mannose, but not glucose or sucrose, disturbs actin cytoskeleton in Arabidopsis thaliana leaves

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Abstract

A dynamic cytoskeleton able to recognize and respond to both abiotic and biotic stimuli is necessary for the proper functioning of a living cell. The cytoskeleton is involved in cell growth and division, maintenance of cell shape, cytoplasmic streaming, and organelle movements. Numerous studies have focused on the relationships between sugar metabolism, sugar signaling, and the cytoskeleton in yeast and animal cells. Data on such connections in plants are scarce. In the present study we investigated the effects of exogenously delivered sugars on the plant actin cytoskeleton. Detached Arabidopsis thaliana leaves were incubated with sugars for 2 days and the cytoskeleton was visualized using fluorescent-labeled phalloidin. Glucose and sucrose did not influence the pattern of the actin cytoskeleton. In contrast, mannose caused the disappearance of filamentous structures and generated actin foci. The symptoms started to be visible after 24 h of the exposure to mannose. The effect did not occur in Nicotiana tabacum mesophyll cells. This insensitivity was probably due to the presence of phosphomannose isomerase in tobacco cells. Mannose is commonly used as a selection marker for the transformation of plants lacking the enzymes responsible for the metabolism of mannose-6-phosphate. Exposure to this hexose has been linked with DNA fragmentation and a release of cytochrome c from mitochondria. Both responses are treated as features of programmed cell death. However, in our experiments no DNA laddering was observed in mannose-treated Arabidopsis leaves.

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Abbreviations

PCD:

Programmed cell death

PMI:

Phosphomannose isomerase

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Acknowledgments

This study was supported by PB 1395/B/P01/2007/33 from Polish Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

    Agnieszka Katarzyna Banaś, Weronika Krzeszowiec & Halina Gabryś

  2. Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

    Jerzy Dobrucki

Authors
  1. Agnieszka Katarzyna Banaś
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  2. Weronika Krzeszowiec
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  3. Jerzy Dobrucki
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  4. Halina Gabryś
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Correspondence to Agnieszka Katarzyna Banaś.

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Communicated by L. A. Kleczkowski.

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Banaś, A.K., Krzeszowiec, W., Dobrucki, J. et al. Mannose, but not glucose or sucrose, disturbs actin cytoskeleton in Arabidopsis thaliana leaves. Acta Physiol Plant 32, 773–779 (2010). https://doi.org/10.1007/s11738-010-0462-5

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  • DOI: https://doi.org/10.1007/s11738-010-0462-5

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