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Hormone and sugar effects on rice sucrose transporter OsSUT1 expression in germinating embryos

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Abstract

The rice (Oryza sativa L.) OsSUT1 gene encodes a sucrose transporter protein. OsSUT1 was suggested to contribute to phloem loading of sucrose. OsSUT1 expression is highly induced in embryos after seeds were imbibed in water and peaked at 2 days after imbibition, but mRNA levels decline gradually afterwards. In this study, we demonstrated that phytohormones and sugars regulate OsSUT1 expression. Antagonism of abscisic acid and gibberellic acid appeared to play an important role in regulating OsSUT1 expression during embryo germination. In addition, our data showed a glucose and sucrose effect on OsSUT1 expression that represented a bi-phase process. Initially, glucose and sucrose functioned as negative regulators of OsSUT1 expression in germinating embryos after a 1-day treatment; however, when the treatment duration was extended to 5 days, OsSUT1 expression was significantly enhanced. Therefore, we hypothesized that the glucose and sucrose effect might occur in combination with other side effects, such as changes in hormone content or catabolism. Based on the effects that sugar analogs have on OsSUT1 expression, we suggest that the signal transduction for regulating glucose-responsive OsSUT1 expression in embryos occurs via a hexokinase-mediated pathway.

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Abbreviations

ABA:

Abscisic acid

DAI:

Days after imbibition

GA:

Gibberellic acid

NAG:

N-acetyl-glucosamine

3-OMG:

3-O-methylglucose

QRT–PCR:

Quantitative real-time reverse transcriptase polymerase chain reaction

SUT:

Sucrose transporter

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Acknowledgments

We are very grateful to Professor Ching-Huei Kao (National Taiwan University, Taiwan) for providing the equipment for analyzing ABA content. We thank Dr. Chih-Wen Wu and Mr. Dah-Pyng Shung for providing rice seeds. This research was supported by a grant from the National Science Council in Taiwan.

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, No. 1, Sect. 4, Roosevelt Rd., Taipei, 106, Taiwan

    Jia-Yi Chen, Shiang-Lin Liu, Wei Siao & Shu-Jen Wang

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  1. Jia-Yi Chen
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  2. Shiang-Lin Liu
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  3. Wei Siao
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  4. Shu-Jen Wang
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Correspondence to Shu-Jen Wang.

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Communicated by J. van Huylenbroeck.

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Chen, JY., Liu, SL., Siao, W. et al. Hormone and sugar effects on rice sucrose transporter OsSUT1 expression in germinating embryos. Acta Physiol Plant 32, 749–756 (2010). https://doi.org/10.1007/s11738-009-0459-0

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  • DOI: https://doi.org/10.1007/s11738-009-0459-0

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