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Effects of exogenous abscisic acid on some physiological responses in a popular aromatic indica rice compared with those from two traditional non-aromatic indica rice cultivars

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Abstract

The poor productivity and local confinement of indigenous aromatic rice varieties are mostly due to their susceptibility to salinity/drought/abscisic acid (ABA)-mediated abiotic stresses. It is thus essential to study the effects of several stress factors on their physiological parameters so as to improve their tolerance mechanism and enhance their global demand. Previously, we studied the effect of salinity stress on the physiological and molecular responses of the common aromatic rice Gobindobhog. The objective of this study was to understand the influence of exogenous ABA on some biochemical parameters in Gobindobhog, and comparison with those from non-aromatic M-1-48 and Nonabokra rice. The highest endogenous hydrogen peroxide content and membrane lipid peroxidation (increased malondialdehyde and lipoxygenase activity) were found in ABA-treated Gobindobhog leaves. While the catalase activity was down regulated the most in ABA-treated Gobindobhog leaves, the guaiacol peroxidase activity was induced maximally, indicating the protective role of peroxidase rather than catalase, during ABA-induced oxidative damages. The antioxidant, anthocyanin, showed the highest level in ABA-treated Nonabokra. Enhanced cysteine, following ABA exposure and the highest levels of reducing sugars, total amino acids, proline, and polyamines (putrescine and spermidine) recorded in Gobindobhog, probably served to shield from ABA-induced stress injuries, whereas the spermine levels were comparable in ABA-treated Nonabokra and Gobindobhog. The aroma content, intensified after ABA treatment, was markedly noted in Gobindobhog. Thus, the systematic examination of ABA-mediated stress revealed the most prominent oxidative damages in Gobindobhog, even higher than M-1-48, with a concomitant enhancement in peroxidase system and particularly osmolyte or polyamine levels to ensure its sustenance.

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Acknowledgments

We gratefully acknowledge the excellent technical assistance of Mr. Jadab Ghosh. This investigation was financially supported by a research grant from the Department of Biotechnology (DBT), Government of India, New Delhi (Grant No.BT/PR2965/AGR/02/155/04/2002) in a National Network Project. ARC acknowledges DBT for providing the award of Senior Research Fellowship during the course of this work.

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  1. Aryadeep Roychoudhury

    Present address: Plant Molecular Biology and Biotechnology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India

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  1. Department of Botany, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata, 700 009, West Bengal, India

    Aryadeep Roychoudhury, Supratim Basu & Dibyendu N. Sengupta

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Roychoudhury, A., Basu, S. & Sengupta, D.N. Effects of exogenous abscisic acid on some physiological responses in a popular aromatic indica rice compared with those from two traditional non-aromatic indica rice cultivars. Acta Physiol Plant 31, 915–926 (2009). https://doi.org/10.1007/s11738-009-0305-4

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