Abstract
The aim of the present work was to study the effect of auxin physiological analogue TA-12 [1-(2-chloroethoksicarbonylmethyl)-4-naphthalenesulfonic acid calcium salt] on the formation of oilseed rape lateral root and on the mitotic activity of apical meristem cells. Spring oilseed rape (Brassica napus L. ssp. oleifera annua Metzg.) cultivar ‘Mascot’ was chosen as a test object. Anatomical, cytological and histological studies on root development suggest that compound TA-12 induces the activity of parent root pericycle cells, stimulates the formation of lateral roots and enhances the division of apical meristem cells. The auxin transport inhibitor 2,3,5-triiodobenzoic acid suppresses the division of apical meristem cells, while this process is restored by the auxin physiological analogue TA-12 and naphthaleneacetic acid. The compound TA-12, by stimulating primary root growth and lateral root induction, optimised the formation of the oilseed rape root system.
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References
Bhalerao R, Eklöf J, Ljung K, Marchant A, Bennett M, Sandberg G (2002) Shoot derived auxin is essential for early lateral root emergence in Arabidopsis seedlings. Plant J 29:325–332
Casimiro I, Marohant A, Bhalero RP, Beeckmann T, Dhoope S, Swarup R, Graham N, Inze D, Sandberg G, Casero PJ, Bennett M (2001) Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell 13:843–852
Choi YE, Katsumi M, Sano H (2001) Triiodobenzoic acid, an auxin polar transport inhibitor, suppresses somatic embryo formation and postembryonic shoot/root development in Eleutherococcus senticosus. Plant Sci 160:1183–1190
Darginavičienė J, Novickienė L (2002) The modern problems of plant physiology. Press of Lithuanian Academy of Sciences, Vilnius, p 99 (in Lithuanian)
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JMS, Jacqmard A, Kilby NJ, Murray JAH (2003) Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3. Plant Cell 15:79–92
Gavelienė V, Novickienė L, Miliuvienė L, Brazauskienė I, Kazlauskienė D (2005) Possibilities to use growth regulators in winter oilseed rape growing technology 2. Effects of auxin analogues on the formation of oilseed rape generative organs and plant winterhardiness. Agron Res Estonia 3(1):9–19
Ivanov VB (1989) Advancement of D.A. Sabinin’s ideas in studies of root growth and morphogenesis. Physiol Plant 36(5):871–879 (in Russian)
Kublickienė O (1978) Histological technique and practical histochemistry. Vilnius, Mokslas, pp 24–41 (in Lithuanian)
Lynch J (1995) Root architecture and plant productivity. Plant Phys 109:7–13
Muday GK, Haworth P (1994) Tomato root growth, gravitropism and lateral development: correlation with auxin transport. Plant Physiol Biochem 32:193–203
Novickienė L (1995) Physiological principles of growth and productivity modification of plant organs by applying new analogues of phytohormones. Biologija 3–4:18–20
Novickienė L, Gavelienė V (2000) Modification of spring rape growth and morphogenesis by physiological analogues of auxin. Sodininkystė ir daržininkystė. Mokslo darbai 19(3)-1:180–192
Novickienė L, Raklevičienė D, Gavelienė V, Kazlauskienė D (2001) The effect of physiological analogues of auxin on in vitro morphogenesis of oilseed rape. Latvian Acad Sci Sect B 55(5/6):201–206
Novickienė L, Gavelienė V, Miliuvienė L, Brazauskienė I, Kazlauskienė D, Pakalniškytė L (2004) New factors in winter rape technology. Biologija 2(2 priedas):91–94
Paulauskas A, Slopšytė G, Morkūnas V (2003) Cell division. In: Methods and practicals of general genetic investigations. pp 22–27 (in Lithuanian)
Reed RC, Brady SR, Muday GK (1998) Inhibition of auxin movement from the shoot into the root inhibits lateral root development in Arabidopsis. Plant Physiol 118:1369–1378
Rubinigg M, Wenisch J, Elzenga JTM, Stulen I (2004) NaCI salinity affects lateral root development in Plantago maritima. Funct Plant Biol 3:775–780
Ruegger M, Deway E, Hobbie L, Brown D, Bernasconi P, Turner J, Muday G, Estelle M (1997) Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with reduction in polar auxin transport and diverse morphological defects. Plant Cell 9:745–757
Songailienė A, Ženauskas K (1985) The biometric evolution of investigation data. Vilnius, Mokslas, p 168 (in Lithuanian)
Teale WD, Paponov IA, Ditengou F, Palme K (2005) Auxin and the developing root of Arabidopsis thaliana. Physiol Plant 123:130–138
Vanneste S, Maes L, Ive DS, Himanen K, Naudts M, Inze D, Beeckman T (2005) Auxin regulation of cell cycle and its role during lateral root initiation. Physiol Plant 123:139–146
Welander M, Zhu LH (2000) The rooting ability of rolB transformed clones of the apple rootstock M26 and its relation to gene expression. Acta Hortical 521:133–138
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This study was supported in part by the Lithuanian State Science and Studies Foundation (‘ABIOTECHA’ project).
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Gavelienė, V., Novickienė, L. & Miliuvienė, L. Improving of oilseed rape lateral root formation by physiological analogues of auxin. Acta Physiol Plant 29, 291–295 (2007). https://doi.org/10.1007/s11738-007-0037-2
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DOI: https://doi.org/10.1007/s11738-007-0037-2