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Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants (Solanum tuberosum L.)

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Abstract

Ascorbate peroxidase (APX) is one of the key enzymes of the plant antioxidant system playing, along with catalase, a central role in hydrogen peroxide scavenging. An approach to further increase the knowledge about cytosolic APX gene organization can be achieved by isolating and characterisating new cDNAs, thus providing new insights about the physiological roles and regulation of these enzymes. A partial cDNA clone (corresponding to the 3’ untranslated region), cytosolic ascorbate peroxidase-related, was isolated from potato sprouts by RT-PCR. Database analysis retrieved several expressed sequence tags (ESTs) coding potato cytosolic ascorbate peroxidase, that were used to infer the complete cDNA sequence. The deduced amino acid sequence revealed high homologies with other plant cytosolic ascorbate peroxidases, confirming the reliability of the virtual cDNA. Northern blot analysis revealed the existence of a single band related to the isolated cDNA and the southern blotting results allowed the elaboration of a possible gene organization.

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Abbreviations

APX:

ascorbate peroxidase

cAPX:

cytosolic APX

Dig:

digoxigenin

DNA:

deoxyribonucleic acid

EST:

expressed sequence tag

H2O2 :

hydrogen peroxide

PCR:

polymerase chain reaction

RNA:

ribonucleic acid

ROS:

reactive oxygen species

RT:

reverse transcription

UV:

ultra violet

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Authors and Affiliations

  1. Plant Functional Biology Department, Institute for Cell and Molecular Biology, University of Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal

    Diana Soares da Costa, Cláudia Sofia Pereira, Jorge Teixeira & Susana Pereira

  2. Botany Department, School of Sciences, University of Porto Rua do Campo Alegre, 1191, 4150-181, Porto, Portugal

    Jorge Teixeira & Susana Pereira

Authors
  1. Diana Soares da Costa
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  2. Cláudia Sofia Pereira
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  3. Jorge Teixeira
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  4. Susana Pereira
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Correspondence to Diana Soares da Costa.

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Soares da Costa, D., Pereira, C.S., Teixeira, J. et al. Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants (Solanum tuberosum L.). Acta Physiol Plant 28, 41–47 (2006). https://doi.org/10.1007/s11738-006-0067-1

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  • DOI: https://doi.org/10.1007/s11738-006-0067-1

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