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Benzopyrans in Hypericum polyanthemum Klotzsch ex Reichardt cultured in vitro

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Abstract

Hypericum polyanthemum Klotzsch ex Reichardt, an endemic species of Southern Brazil, was micropropagated on MS medium supplemented with 1.78 μM BAP. Shoot proliferation and rooting was achieved on hormone-free medium and plantlets survived acclimatization. The bioactive compounds: 6-isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran (HP1), 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran (HP2) and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) were quantified in the leaves, stems and roots of propagated and acclimatized plantlets and compared with the field-grown plants. The HPLC analysis revealed that the three benzopyrans are accumulated in the aerial parts and the concentration varied with the age of the plant whereas the roots were capable of accumulating only HP3. Greatest yield of HP1 (7.12 mg/g DW) was quantified in the leaves of the acclimatized plantlets, whereas the flowers of the plants from natural habitat displayed higher amounts of HP2 (11.04 mg/g DW) and HP3 (13.99 mg/g DW).

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Abbreviations

BAP:

6-Benzylaminopurine

HP1:

6-Isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran

HP2:

7-Hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran

HP3:

5-Hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran

HPLC:

High performance liquid chromatography

IAA:

Indole-3-acetic acid

DW:

Dry weight

MS:

Murashige and Skoog

NAA:

α-Naphthalene-acetic acid

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Acknowledgments

This study was supported by CNPq, FAPERGS and Propesq-UFRGS. The authors thank CBIM/UFRGS for the HPLC facilities.

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Authors and Affiliations

  1. Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, 90610-000, Porto Alegre, Brazil

    Ana Paula M. Bernardi, Natasha Maurmann, Sandra B. Rech & Gilsane von Poser

Authors
  1. Ana Paula M. Bernardi
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  2. Natasha Maurmann
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  3. Sandra B. Rech
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  4. Gilsane von Poser
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Correspondence to Gilsane von Poser.

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Communicated by S. Lewak.

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Bernardi, A.P.M., Maurmann, N., Rech, S.B. et al. Benzopyrans in Hypericum polyanthemum Klotzsch ex Reichardt cultured in vitro. Acta Physiol Plant 29, 165–170 (2007). https://doi.org/10.1007/s11738-006-0021-2

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  • DOI: https://doi.org/10.1007/s11738-006-0021-2

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