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Early Postoperative Changes of HDL Subfraction Profile and HDL-Associated Enzymes After Laparoscopic Sleeve Gastrectomy

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Abstract

This study aimed to determine early postoperative changes of LDL/HDL subfraction profile and HDL-associated enzymes following laparoscopic sleeve gastrectomy (LSG). Thirteen obese patients (mean body mass index (BMI) 52.74 ± 10.97 kg/m2) underwent LSG and normal weight control patients (mean BMI 23.56 ± 1.92 kg/m2) underwent laparoscopic abdominal surgery. Fasting blood samples were collected prior to surgery, at day 1 after surgery, and after postoperation oral feeding. LDL and HDL subfraction analysis was done by continuous disk polyacrylamide gel electrophoresis. Plasma levels of cholesteryl ester transfer protein (CETP), lecithin–cholesterol acyltransferase (LCAT), and apolipoprotein A-1 (apoA-I) were determined by enzyme-linked immunosorbent assay. Measurement of CETP and LCAT activity was performed via fluorometric analysis. LDL subfraction profile showed no change in both LSG and control group patients. No significant difference was observed in HDL cholesterol, HDL-subfraction distribution, and apoA-I levels in the control group. LSG patients showed a significant increase in HDL-large and a significant decrease in HDL-small fractions at postoperation day 1 compared to preoperation. HDL cholesterol significantly decreased and apoA-I significantly increased in LSG patients after postoperation oral feeding compared to both preoperation and postoperation day 1. Changes in HDL subfraction profile at postoperation day 1 after LSG were accompanied by a significant decrease in CETP protein, LCAT protein, and LCAT activity as compared to preoperation levels. Early changes in HDL subfraction profile and HDL-associated enzymes following LSG suggest that the surgical procedure, irrespective of changes in body weight, affects reverse cholesterol transport.

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Acknowledgments

This study was supported by a grant (No. 2010.04.0103.014) from Akdeniz University Research Foundation.

Conflict of Interest

All authors declare that they have no financial, consulting, and personal relationships with other people or organizations that could influence the presented work.

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Authors and Affiliations

  1. Department of Biochemistry, Akdeniz University Medical School, 07070, Antalya, Turkey

    Serdar Doğan & Mutay Aslan

  2. Endocrinology Clinic, Antalya Research and Education Hospital, 07100, Antalya, Turkey

    Ibrahim Aslan

  3. Surgery Clinic, Antalya Research and Education Hospital, 07100, Antalya, Turkey

    Ramazan Eryılmaz, Cemal Ozben Ensari & Tuna Bilecik

Authors
  1. Serdar Doğan
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  2. Ibrahim Aslan
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  3. Ramazan Eryılmaz
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  4. Cemal Ozben Ensari
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  5. Tuna Bilecik
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  6. Mutay Aslan
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Correspondence to Mutay Aslan.

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Doğan, S., Aslan, I., Eryılmaz, R. et al. Early Postoperative Changes of HDL Subfraction Profile and HDL-Associated Enzymes After Laparoscopic Sleeve Gastrectomy. OBES SURG 23, 1973–1980 (2013). https://doi.org/10.1007/s11695-013-1011-2

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  • DOI: https://doi.org/10.1007/s11695-013-1011-2

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