Abstract
Kenaf (Hibiscus cannabinus L.) is a fast-growing herbaceous plant that received great attention in Malaysia as a valuable fibre crop. Yet, different maturity of plants’ leaves could affect the antioxidant capacities of the tea prepared. Therefore, the aim of this study was to determine the physical properties of KR9 kenaf leaves and antioxidant activities of tea prepared from KR9 kenaf leaves at four different maturity stages, which were 60, 90, 120 and 150 days after sowing (DAS). The analysis that carried out were DPPH (2-2-diphenyl-1-picrylhydrazyl) radical scavenging assay, ABTS (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay, ferric reducing antioxidant power assay, total phenolic content assay, total flavonoid content assay, and chromatographic analysis of phenolic compounds. Results demonstrated that the kenaf leaves at 150 DAS were able to retain the most colour after drying. It also revealed that the tea prepared from kenaf leaves at 120 DAS showed the highest antioxidant activity in all antioxidant analysis tested. Kenaf leaves tea contained mainly kaempferol, tannic acid, chlorogenic acid, caffeic acid and catechin hydrate. Hence, it was deduced that the antioxidant activity in kenaf leaves increased until 120 DAS and dropped at 150 DAS. There was a strong positive correlation relationship among all the antioxidant analysis and the phenolic compounds presented in the kenaf leaves tea. Thus, the phenolic compounds detected contributed significantly to the antioxidant activities of the kenaf leaves tea. The 120 DAS kenaf leaves was recommended for tea preparation since it possessed high antioxidant activities.
Similar content being viewed by others
References
K. Izran, A. Zaidon, A.M.A. Rashid, F. Abood, M.J. Saad, M.Z. Thirmizir, K. Masseat, S. Rahim, Asian J. Appl. Sci. 2(5), 446–455 (2009)
S. Zhao, X. Li, D.H. Cho, M.V. Arasu, N.A. Al-Dhabi, S.U. Park, Molecules 19(10), 16987–16997 (2014)
J. Ryu, B. Ha, D. Kim, J. Kim, S. Kim, S. Kang, J. Crop Sci. Biotechnol. 16(4), 297–302 (2013)
A. Pascoal, R. Quirantes-Piné, A.L. Fernando, E. Alexopoulou, A. Segura-Carretero, Ind. Crops Prod. 78, 116–123 (2015)
M.Y. Masnira, R.A. Halim, M.Y. Rafii, J. Mohd, M.Y. Martini, J. ISSAAS. 21(2), 29–142 (2015)
National Kenaf and Tobacco Board, Kenaf Introduction in Malaysia. (LKTN, 2018), http://www.forumue.de/wp-content/uploads/2018/03/2018_02_10_malaysia_engl_web.pdf
Z. Cheng, B.R. Lu, B.S. Baldwin, K. Sameshima, J.K. Chen, Hereditas. 136(3), 231–239 (2002)
C. Quispe, E. Viveros-Valdez, G. Schmeda-Hirschmann, Plant Foods Hum. Nutr. 67(3), 242–246 (2012)
F. Dian-Nashiela, A. Noriham, H. Nooraain, A.H. Azizah, Int. Food Res. J. 22(3), 1189–1194 (2015)
S. Seena, K.R. Sridhar, A.B. Arunb, C.C. Young, J. Food Comp. Anal. 19, 284–293 (2006)
K.W. Chan, M. Ismail, Food Chem. 114(3), 970–975 (2009)
C.L. Webber III, V.K. Bledsoe, Ind. Crops Prod. 16(2), 81–88 (2002)
S.S. Kumar, P. Manoj, N.P. Shetty, P. Giridhar, J. Sci. Food Agric. 95(9), 1812–1820 (2015)
K.E. Ileleji, A.A. Garcia, A.R. Kingsly, C.L. Clementson, J. AOAC Int. 93(3), 825–832 (2010)
J.J.Y. Tan, Y.Y. Lim, L.F. Siow, J.B.L. Tan, J. Food Process. Preserv. 39(6), 2811–2819 (2015)
Q. Meng, H. Fan, Y. Li, L. Zhang, J. Food Meas. Charact. 12(1), 1–10 (2018)
C.W. Jin, A.K. Ghimeray, L. Wang, M.L. Xu, J.P. Piao, D.H. Cho, J. Med. Plants Res. 7(17), 1121–1128 (2013)
A. Floegel, D.O. Kim, S.J. Chung, S.L. Koo, O.K. Chun, J. Food Compos. Anal. 24(7), 1043–1048 (2011)
P.C. Wootton-Beard, A. Moran, L. Ryan, Food Res. Int. 44(1), 217–224 (2011)
Y.Y. Lim, T.T. Lim, J.J. Tee, Food Chem. 103(3), 1003–1008 (2007)
E. Verzelloni, D. Tagliazucchi, A. Conte, Food Chem. 105(2), 564–571 (2007)
Y.H. Wong, H.W. Lau, C.P. Tan, K. Long, K.L. Nyam, Sci. World J. 2014, 789346 (2014)
C.L. Webber III, H.L. Bhardwaj, V.K. Bledsoe, Trends in New Crops and New Uses (ASHS Press, Alexandria, 2002), pp. 327–339
T. Yamamoto, L. Juneja, S. Chu, M. Kim, Chemistry and Applications of Green Tea (CRC Press, Boca Raton, 1997), p. 6
A. Karim, C. Law, Intermittent and Nonstationary Drying Technologies (CRC Press, Boca Raton, 2017), p. 76
R. Arias, T.C. Lee, L. Logendra, H. Janes, J. Agric. Food Chem. 48(5), 1697–1702 (2000)
M. Zhang, N.S. Hettiarachchy, R. Horax, P. Chen, K.F. Over, J. Food Sci. 74(6), 441–448 (2009)
M. Youssef, M. Mokhtar, J. Nutr. Food Sci. 4(6), 309 (2014)
M. Braga, E. Vieira, T. de Oliveira, Food Chem. 265, 308–315 (2018)
L. Zhou, Y. Wang, X. Hu, J. Wu, X. Liao, Innov. Food Sci. Emerg. Technol. 10(3), 321–327 (2009)
D.B. Rodriguez-Amaya, Forum Nutr. 56, 35–37 (2003)
A. Bahtoee, K. Zargari, E. Baniani, World Appl. Sci. J. 16(1), 63–66 (2012)
F.M. Oloyede, F.A. Oloyede, E.M. Obuotor, Bull. Environ. Pharmacol. Life Sci. 2(2), 18–21 (2013)
P.E.T.R.A. Zimmermann, U.L.R.I.K.E. Zentgraf, Cell. Mol. Biol. Lett. 10(3), 515–534 (2005)
R. Farhoosh, G.A. Golmovahhed, M.H. Khodaparast, Food Chem. 100(1), 231–236 (2007)
C.W. Jin, S.H. Eom, H.J. Park, A.K. Ghimeray, C.Y. Yu, D.H. Cho, Korean J. Med. Crop Sci. 17(1), 21–25 (2009)
V. Khader, S. Rama, Korean J. Med. Crop Sci. 17(1), 21–25 (1998)
J. Ryu, S.J. Kwon, J.W. Ahn, Y.D. Jo, S.H. Kim, S.W. Jeong, M.K. Lee, J.B. Kim, S.Y. Kang, J. Plant Biotechnol. 44(2), 191–202 (2017)
Y.H. Wong, J.S. Beh, C.P. Tan, K. Long, K.L. Nyam, Free Radic. Antioxid. 4(2), 8–12 (2014)
M.F.A. Bakar, M. Mohamed, A. Rahmat, J. Fry, Food Chem. 113(2), 479–483 (2009)
J.J. Ling, D. Maryati Mohamed, A. Rahmat, M.F.A. Bakar, J. Med. Plant Res. 4(1), 27–32 (2010)
V. Katalinic, M. Milos, T. Kulisic, M. Jukic, Food Chem. 94(4), 550–557 (2006)
C.C. Wong, M.M. Daham, O. Abdullah, J. Trop. Agric. Food. Sci. 36(1), 21–28 (2008)
S. Dudonné, X. Vitrac, P. Coutiere, M. Woillez, J.M. Mérillon, J. Agric. Food Chem. 57(5), 1768–1774 (2009)
Acknowledgements
Financial support of this work by the Ministry of Higher Education through the Fundamental Research Grant Scheme (FRGS/1/2018/WAB01/UCSI/02/1) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Kho, K., Sim, Y.Y. & Nyam, K.L. Antioxidant activities of tea prepared from kenaf (Hibiscus cannabinus L. KR9) leaves at different maturity stages. Food Measure 13, 2009–2016 (2019). https://doi.org/10.1007/s11694-019-00121-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-019-00121-8