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HPTLC–MS based method development and validation for the detection of adulterants in spices

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Abstract

Development and validation of high performance thin layer chromatographic method was carried out for the estimation of adulterants (sudan dyes) in turmeric and chili powdered spices. The separation of sudan dyes were obtained over aluminium baked HPTLC plates pre-coated with silica gel RP-18 F254 as stationary phases with optimized triphasic mobile phase acetonitrile: acetone: ammonia (9.0:0.5:0.5; v/v/v) with 5 min saturation time. Validation was carried out by testing its specificity, linearity, accuracy, inter and intraday precision, repeatability, limit of detection and limit of quantification according to ICH guidelines. Recording of characteristic molecular ion peaks of sudan I, II, III and IV at 248, 278, 353 and 381, respectively highlights the use of TLC–MS interface confirming the adulteration of sudan dyes in some commercially available powders of spices.

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Acknowledgments

The authors are grateful to Prof. P K Kalra, Director, Prof. Sahab Dass, Head, Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra. The authors are also thankful to Ministry of Human Resource and Development, Govt. of India, under Grant MHRD-NMEICT scheme entitled ‘MHRD Virtual Chemistry Lab’ No. F. 16-35/2009DL, for rendering financial assistance.

Conflict of interest

Roopa Rani, Sharad Medhe and M. M Srivastava declare that they have no conflict of interest and are grateful to Ministry of Human Resource and Development, Govt. of India, for rendering financial assistance.

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Authors and Affiliations

  1. Department of Chemistry, Dayalbagh Educational Institute, Agra, 282110, India

    Roopa Rani, Sharad Medhe & ManMohan Srivastava

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  1. Roopa Rani
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  2. Sharad Medhe
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  3. ManMohan Srivastava
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Correspondence to ManMohan Srivastava.

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Rani, R., Medhe, S. & Srivastava, M. HPTLC–MS based method development and validation for the detection of adulterants in spices. Food Measure 9, 186–194 (2015). https://doi.org/10.1007/s11694-015-9223-x

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  • DOI: https://doi.org/10.1007/s11694-015-9223-x

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