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Influence of cytokinins, basal media and pH on adventitious shoot regeneration from excised root cultures of Albizia lebbeck

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Abstract

A highly reproducible and efficient in vitro shoot regeneration system was developed in a potential medicinal plant, Albizia lebbeck using root explants. Root explants from 15 day-old-aseptic seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5 and 10.0 μM) of 6-Benzyladenine (BA), Kinetin (Kn), 2-Isopentenyl adenine (2-iP) singly as well as in combination with α-Naphthalene acetic acid (NAA) (0.1, 0.5, 1.0, 1.5 and 2.0 μM). The highest rate of shoot multiplication (16.0 ± 1.87 for the average shoot number and 5.16 ± 0.38 cm for shoot length) was achieved on MS medium supplemented with 7.5 μM BA and 0.5 μM NAA. The effects of medium type, medium strength, pH and subculture on shoot induction and proliferation were also tested. An average of 21.6±2.87 shoots per explants could be obtained following this protocol. Rooting was achieved on microshoots using half strength MS medium with 2.0 μM Indole-3-butyric acid (IBA) after four weeks of culture. The in vitro raised healthy plantlets were successfully established in earthen pots containing garden soil and grown in greenhouse with >80% survival rate.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Shahnaz Perveen, Ankita Varshney & Mohammad Anis

  2. Department of Plant Production, College of Food & Agricultural Sciences, King Saud University, P.O Box 2460, Riyadh, 11451, Saudi Arabia

    Mohammad Anis & Ibrahim M. Aref

Authors
  1. Shahnaz Perveen
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  2. Ankita Varshney
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  3. Mohammad Anis
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  4. Ibrahim M. Aref
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Correspondence to Mohammad Anis.

Additional information

Foundation project: This work is financially supported from the DSTFIST (2005–2010) and UGC-SAP (DRS-I) Programmes (2009–2014), Govt of India, New Delhi.

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Perveen, S., Varshney, A., Anis, M. et al. Influence of cytokinins, basal media and pH on adventitious shoot regeneration from excised root cultures of Albizia lebbeck . Journal of Forestry Research 22, 47–52 (2011). https://doi.org/10.1007/s11676-011-0124-5

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  • DOI: https://doi.org/10.1007/s11676-011-0124-5

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