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Relationship between osteoporosis and benign paroxysmal positional vertigo based on evidence-based medicine and bioinformatics

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Abstract

Summary

It has been reported that osteoporosis is a possible risk factor of benign paroxysmal positional vertigo (BPPV).

Purpose

We analyzed the correlation between osteoporosis and BPPV and the possible mechanism by performing evidence-based medicine meta-analysis and bioinformatics analysis.

Methods

Initially, English articles related to osteoporosis and BPPV were obtained through PubMed and EMBASE databases. Stata12.0 software was used for meta-analysis to calculate the odd ratio (OR) and 95% confidence interval (CI) of outcome indicators, and the heterogeneity was evaluated by subgroup analysis, publication bias evaluation, and sensitivity analysis. In addition, microarray datasets related to BPPV and osteoporosis were obtained from gene expression omnibus (GEO) database to screen differentially expressed genes. At last, a mouse model of osteoporosis was established by bilateral oophorectomy for validation. RT-qPCR and Western blot analysis were performed to determine expression of related factors in mouse tissues.

Results

Osteoporosis was suggested as an important risk factor for BPPV through meta-analysis of these 12 articles. It was found that PPP2CA was upregulated in BPPV and low bone mineral density (BMD) samples. Moreover, PPP2CA induced dephosphorylation of BCL2, which may be involved in BPPV through regulation of BMD. Through this mechanism, silencing of PPP2CA could elevate the incidence of BPPV by promoting bone remodeling and reducing the density of otoconia around the macula.

Conclusions

PPP2CA reduces BMD expression by inducing dephosphorylation of BCL2, which may be one of the mechanisms responsible for the onset of BPPV in osteoporosis.

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Data availability

Available upon request.

Code availability

Not applicable.

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Funding

This study was supported by the Shaanxi Administration of Traditional Chinese Medicine Project in 2019 (2019-GJ-LC003), Xianyang Young and Middle-aged Science and Technology Innovation Leading Talents Project in 2018 [Xiankefa (2018) 64], and Key Innovation Project of Xianyang Central Hospital in 2019 (2019Z-02).

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Authors and Affiliations

  1. Department of Orthopedics, Xianyang Central Hospital, Xianyang, 712000, People’s Republic of China

    Tuanmao Guo, Haiyun Zhu, Lan Yang, Yuan Xiao & Jiang Xu

  2. Department of Pharmacy, Xianyang Central Hospital, Shanxi Province, No. 78, Renmin East Road, Xianyang, 712000, People’s Republic of China

    Yanli Xing

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  3. Haiyun Zhu
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Contributions

Tuanmao Guo and Yanli Xing designed the study. Tuanmao Guo, Haiyun Zhu, and Lan Yang collated the data, carried out data analyses, and produced the initial draft of the manuscript. Yuan Xiao and Jiang Xu contributed to drafting the manuscript. All authors have read and approved the final submitted manuscript.

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Correspondence to Yanli Xing.

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Guo, T., Xing, Y., Zhu, H. et al. Relationship between osteoporosis and benign paroxysmal positional vertigo based on evidence-based medicine and bioinformatics. Arch Osteoporos 16, 173 (2021). https://doi.org/10.1007/s11657-021-01006-4

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