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Anti-inflammatory Effects of Ampelopsis Japonica Root on Contact Dermatitis in Mice

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Abstract

Objective

To investigate the anti-inflammatory potential of Ampelopsis japonica on contact dermatitis (CD).

Methods

A total of 38 Balb/c mice were divided into 5 groups by using a random number table: normal mice (n=6), CD model mice (n=8), CD mice treated with 3 or 30 mg/kg of the ethanol extract of A. japonica (EEAJ, n=8) and 7.5 mg/kg dexamethasone treated CD mice (DEX, n=8). CD was induced using topical application of 1-fluoro-2,4-dinitrofluorobenzene in mice. EEAJ and DEX were topically applied to the shaved skin of each mouse for 6 days, and the effects of EEAJ and DEX on skin lesions and color, histopathological abnormalities such as epidermal hyperplasia and immune cell infiltration, and tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) production were investigated. The effects on changes in body weights and spleen/body weight ratio were also investigated.

Results

EEAJ at 30 mg/kg significantly prevented scaling, erythema and enlargement of skin weight compared to using carbon dioxide. EEAJ also prevented epithelial hyperplasia and immune cell infiltrations induced by repeated application of DNFB (P<0.01). In addition, EEAJ significantly lowered levels of TNF-α, IL-6 and MCP-1 (P<0.05 or P<0.01). The anti-inflammatory effects of EEAJ were similar to those of DEX.

Conclusion

A. japonica may be a new therapeutic agent with the potential to reduce or replace corticosteroids and its mechanisms are closely related to regulation of TNF-α production.

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Authors and Affiliations

  1. Division of Pharmacology, School of Korean Medicine, Pusan National University, Gyeongnam, 50612, South Korea

    Yoona Oh, Hongbum Lee, Beodeul Yang, Sura Kim & Hyungwoo Kim

  2. Department of Pathology, College of Korean Medicine, Dongshin University, Jeonnam, 58245, South Korea

    Hyunwoo Jeong

Authors
  1. Yoona Oh
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  2. Hongbum Lee
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  3. Beodeul Yang
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  4. Sura Kim
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  5. Hyunwoo Jeong
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  6. Hyungwoo Kim
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Contributions

Conception and study design: Kim H, Jeong H; funding obtaining: Kim H; data acquisition: Oh Y, Lee H, Yang B and Kim S; data analysis and interpretation: Kim H and Jeong H; statistical expertise: Kim H; administrative technical/logistic support, data collection and assembly: Oh Y, Lee H and Kim H; critical revision of the article for important intellectual content: Kim H and Jeong H; article drafting and final approval: all authors.

Corresponding author

Correspondence to Hyungwoo Kim.

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Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this article.

Supported by the National Research Foundation of Korea funded by the Korean Government (MSIP; No. 2018R1A2B6006031)

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Oh, Y., Lee, H., Yang, B. et al. Anti-inflammatory Effects of Ampelopsis Japonica Root on Contact Dermatitis in Mice. Chin. J. Integr. Med. 28, 719–724 (2022). https://doi.org/10.1007/s11655-022-3517-0

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  • DOI: https://doi.org/10.1007/s11655-022-3517-0

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