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Protective effect of salidroside on contrast-induced nephropathy in comparison with N-acetylcysteine and its underlying mechanism

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Abstract

Objective

To study the prevention effect of salidroside on contrast-induced-nephropathy (CIN) and its underlying mechanism.

Methods

A total of 24 Wistar rats were randomly divided into 4 groups with 6 in each group. Rats were firstly administrated with normal saline (control and model groups), N-acetylcysteine (NAC, NAC group) and salidroside (salidroside group) for 7 days before model establishment in each group, respectively. Histopathological analysis was performed by periodic acid-Schiff (PAS) staining. Oxidative stress related parameters including superoxide dismutase (SOD) and methane dicarboxylic aldehyde (MDA), nitric oxide (NO), angiotensin II (Ang II), 8-hydroxy-2′-deoxyguanosine (8-OHdG), mRNA and protein levels of endothelial nitric oxide synthase (eNOS), and nitric oxide synthase (NOS) activity were measured.

Results

Compared with the control group, the levels of MDA, Ang II and 8-OHdG were all significantly increased and levels of SOD, NO, and eNOS mRNA and protein were decreased significantly in the model group (P<0.05). Meanwhile, the NOS activity was also significantly decreased in the model group (P<0.05). In addition, the levels of these parameters were all improved in the NAC (P<0.05) and salidroside groups and no significant different was found between these two groups (P>0.05).

Conclusion

Salidroside can be the potential substitute of NAC to prevent CIN. The underlying mechanism may be associated with oxidative stress damage caused by contrast agents.

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Author information

Authors and Affiliations

  1. State Discipline and State Key Laboratory of Kidney Disease (Chinese PLA General Hospital, 2011DAV00088), Beijing, 100853, China

    Yue Xing  (邢 悦), Ri-bao Wei  (魏日胞), Yue Yang  (杨 悦), Xiao-yong Zheng  (郑晓勇), Zi-cheng Wang  (王子承) & Yu-wei Gao  (高玉伟)

  2. Department of Rheumatism Immunity, Tianjin First Center Hospital, Tianjin, 300192, China

    Lu Tang  (唐 露)

Authors
  1. Yue Xing  (邢 悦)
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  2. Ri-bao Wei  (魏日胞)
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  3. Lu Tang  (唐 露)
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  4. Yue Yang  (杨 悦)
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  5. Xiao-yong Zheng  (郑晓勇)
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  6. Zi-cheng Wang  (王子承)
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  7. Yu-wei Gao  (高玉伟)
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Corresponding author

Correspondence to Ri-bao Wei  (魏日胞).

Additional information

Supported by the National Nature Science Foundation of China (No. 81273968 and No. 81471027), and Ministerial Project of the National Working Commission on Aging (No. QLB2014W002)

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Xing, Y., Wei, Rb., Tang, L. et al. Protective effect of salidroside on contrast-induced nephropathy in comparison with N-acetylcysteine and its underlying mechanism. Chin. J. Integr. Med. 21, 266–273 (2015). https://doi.org/10.1007/s11655-015-2137-y

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  • DOI: https://doi.org/10.1007/s11655-015-2137-y

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