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Effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice

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Abstract

Objective

To observe the effect of Safflor Yellow (SY) Injection on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.

Methods

Seventy-two mice were divided into six groups: control (saline + saline); LPS (LPS + saline); SY Injection [LPS + SY (10, 20 or 40 mg/kg, intravenously)] and anisodamine (AD) (LPS + AD). Thirty minutes after SY or AD administration, 15 mg/kg LPS was given intraperitoneally. All animals were sacrificed 4 h after LPS injection. Arterial blood gas and lung water content index (LWCI) were measured. Lung tissue myeloperoxidase (MPO) activity was assayed. mRNA expression of inflammatory cytokines was assayed by reverse-transcription polymerase chain reaction. Lung morphological and nuclear factor (NF)-κB p65-positive cell changes were observed by HE and immunohistochemical staining. p38 mitogen-activated protein kinase (MAPK) phosphorylation was observed by Western blotting.

Results

After LPS administration, all animals displayed increased arterial carbon dioxide partial pressure (PaCO2) and decreased arterial oxygen partial pressure (PaO2), arterial oxygen saturation (SO2), HCO3 − concentration and pH, and increased LWCI, MPO activity, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α mRNA expression, NF-κB p65-positive staining and p38 MAPK activation compared with normal controls (all P<0.01). SY Injection significantly mitigated the LPS-induced increase in arterial PaCO and the decreases in arterial PaO2, SO2 and pH, and attenuated increases in LWCI and lung tissue MPO activity (all P<0.01). Moreover, SY Injection inhibited the increases in NF-κB p65 staining and in TNF-α, IL-1β and IL-6 mRNA expression (all P<0.01), and promoted the expression of the antiinflammatory cytokine IL-10 (P<0.05) following LPS injection. LPS-induced pulmonary p38 MAPK phosphorylation was suppressed by pretreatment with SY Injection (P<0.01).

Conclusion

SY Injection ameliorates inflammatory ALI induced by LPS in mice.

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Authors and Affiliations

  1. Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China

    Ming Jin  (金 鸣), Chun-yan Sun  (孙春燕), Chong-qiang Pei  (裴崇强) & Lin Wang  (王 麟)

  2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China

    Pei-cheng Zhang  (张培成)

Authors
  1. Ming Jin  (金 鸣)
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  2. Chun-yan Sun  (孙春燕)
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  3. Chong-qiang Pei  (裴崇强)
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  4. Lin Wang  (王 麟)
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  5. Pei-cheng Zhang  (张培成)
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Corresponding author

Correspondence to Ming Jin  (金 鸣).

Additional information

Supported by National Natural Science Foundation of China (No. 30572344), Natural Science Foundation of Beijing (No. 7102025), and Science and Technology Personnel Serve Enterprise Action (No. 2009GJA30001)

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Jin, M., Sun, Cy., Pei, Cq. et al. Effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice. Chin. J. Integr. Med. 19, 836–843 (2013). https://doi.org/10.1007/s11655-012-1151-6

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  • DOI: https://doi.org/10.1007/s11655-012-1151-6

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