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Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa

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Abstract

The aim of this study was to investigate both a mass in vitro propagation system and the β-ecdysone content in roots and aerial parts of Pfaffia glomerata and Pfaffia tuberosa. Nodal segments of two genotypes (BRA and JB-UFSM) of P. glomerata, originated from aseptically grown plants, were cultivated on hormone-free Murashige and Skoog medium. For the proliferation of P. tuberosa shoots, nodal segments, originated from aseptically grown plants, were either cultivated on hormone-free Murashige and Skoog (MS) medium or were supplemented with 1.0 µM thidiazuron (TDZ); the elongation and rooting of these plants were carried out on MS medium without TDZ. Plantlets of both species were acclimatized and transferred to field conditions. The β-ecdysone content in the plants was determined by high performance liquid chromatography. The BRA genotype showed a greater in vitro proliferation rate and β-ecdysone content than that of the JB-UFSM genotype. The culture of nodal segments of P. tuberosa on medium with 1.0 µM TDZ with subsequent subcultivation of shoots on hormone-free medium was shown to be a suitable method for micropropagation due to the high multiplication rate and good plant development. Both species showed good adaptation to ex vitro conditions. The β-ecdysone content in micropropagated P. tuberosa was similar to that found in field-grown plants. For both species, the aerial parts accumulated higher β-ecdysone content than roots. These results reveal that micropropagation is a successful, alternative method for rapid plant multiplication of both species of Brazilian ginseng. Furthermore, this study demonstrates that these two species have a potential for cultivation that is associated with high β-ecdysone production.

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Acknowledgments

The authors thank Dr. R. Fontes and G. R. de L Fortes (Embrapa Cenargen, Brazil) for the supply of BRA genotype of Brazilian ginseng.

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Authors and Affiliations

  1. Department of Biology, Laboratory of Biotechnology, Universidade Federal de Santa Maria (UFSM), 97105-900, Santa Maria, Rio Grande do Sul, Brazil

    Rejane Flores & Fernando Teixeira Nicoloso

  2. Department of Chemistry, Laboratory of Pharmaceutical and Chemical Analysis, Universidade Regional Integrada (URI), 98400-000, Frederico Westphalen, Rio Grande do Sul, Brazil

    Daniela Brondani Jr, Verciane Cezarotto Jr & Sandro Rogério Giacomelli

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  1. Rejane Flores
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  2. Daniela Brondani Jr
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  3. Verciane Cezarotto Jr
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  4. Sandro Rogério Giacomelli
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  5. Fernando Teixeira Nicoloso
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Correspondence to Fernando Teixeira Nicoloso.

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Editor: N. J. Taylor

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Flores, R., Brondani, D., Cezarotto, V. et al. Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa . In Vitro Cell.Dev.Biol.-Plant 46, 210–217 (2010). https://doi.org/10.1007/s11627-010-9286-7

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