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Polycaprolactone/poly l-lactic acid nanofibrous scaffold improves osteogenic differentiation of the amniotic fluid-derived stem cells

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Abstract

Using stem cells is one of the most important determining factors in repairing lesions using regenerative medicine. Obtaining adult stem cells from patients is a perfect choice, but it is worth noting that their differentiation and proliferation potential decreases as the patient ages. For this reason, the use of amniotic fluid stem cells can be one of the excellent alternatives. This research aimed to investigate the osteogenic differentiation potential of the amniotic fluid stem cells while cultured on the polycaprolactone/poly l-lactic acid nanofibrous scaffold. Scaffolds were qualitatively evaluated by a scanning electron microscope, and their hydrophilicity and mechanical properties were studied using contact angle and tensile test, respectively. The biocompatibility and non-toxicity of the nanofibers were also evaluated using viability assay. The osteo-supportive capacity of the nanofibers was examined using alizarin red staining, alkaline phosphatase activity, and calcium release measurement. Finally, the expression level of four important bone-related genes was determined quantitatively. The results demonstrated that the mineralization rate, alkaline phosphatase activity, intracellular calcium, and bone-related genes increased significantly in the cells cultured on the polycaprolactone/poly l-lactic acid scaffold compared to the cells cultured on the tissue culture plate as a control. According to the results, it can be concluded that the polycaprolactone/poly l-lactic acid nanofibrous scaffold surprisingly improved the osteogenic differentiation potential of the amniotic fluid stem cells and, in combination with polycaprolactone/poly l-lactic acid nanofibers could be a promising candidate as bone implants.

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Data availability

The data supporting this study’s findings are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Seyyedeh Masoumeh Pishnamazi & Shiva Irani

  2. Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sayyed Mohammad Hossein Ghaderian

  3. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abdolreza Ardeshirylajimi

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  1. Seyyedeh Masoumeh Pishnamazi
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  2. Sayyed Mohammad Hossein Ghaderian
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  3. Shiva Irani
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  4. Abdolreza Ardeshirylajimi
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S.M.P.: literature, accomplish experiments, data analysis, and manuscript draft preparation; A.A., S.I., and S.M.H.G.: experiments’ design, data analysis, and data and manuscript approval.

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Correspondence to Sayyed Mohammad Hossein Ghaderian.

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This study was approved by the research committee of the Biology Department of Islamic Azad University, Science and Research Unit, Tehran, Iran.

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Editor: John W. Harbell.

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Pishnamazi, S.M., Ghaderian, S.M.H., Irani, S. et al. Polycaprolactone/poly l-lactic acid nanofibrous scaffold improves osteogenic differentiation of the amniotic fluid-derived stem cells. In Vitro Cell.Dev.Biol.-Animal 60, 106–114 (2024). https://doi.org/10.1007/s11626-023-00838-3

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