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The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress

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Abstract

High ambient temperature is one of the most important environmental factors that caused the reduction of livestock productivity and the increase of mortality. It has been shown that heat stress could affect the meat quality characteristics by physiological and metabolic perturbations in live livestock. Rosmarinic acid (RA) is a natural polyphenolic phytochemical compound that has many important biological activities, such as antioxidant, antimutagenic, and antitumor. The purpose of this study was to investigate the possible function and mechanism of RA on myoblast proliferation and differentiation under heat stress condition. The results showed that heat stress reduced the viability of myoblast and increased the percentage of apoptotic cells, and it also disrupted myotube formation by altering the expression of myogenic regulatory factors MyoD, myogenin, and MyHC. However, pretreatment of RA can protect C2C12 cells from heat stress–induced apoptosis, and it also increased the expression level of MyoD, myogenin, and MyHC under heat stress, which indicated that RA have protective effect on heat stress–caused failure of myotube formation during myoblast differentiation. Above all, our finding demonstrated that RA can promote the differentiation of C2C12 myoblast and maintain the formation of myotubes even under heat stress condition.

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Funding

This work was financially supported by the Natural Science Foundation of Jiangsu Province (grant number BK20190254), the China Postdoctoral Science Foundation (grant number 2019M651755), and the Jiangsu Agricultural Science And Technology Innovation Fund (grant number CX(19)3021).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Kun-Lin Chen

  2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Yue Wang & Hui-Xia Li

  3. Youyuan Research Institute of Dairy Industry Co., Ltd, Nanjing, 211100, China

    Zhi-Ping Lin

Authors
  1. Kun-Lin Chen
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  2. Yue Wang
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  3. Zhi-Ping Lin
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  4. Hui-Xia Li
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Correspondence to Hui-Xia Li.

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Editor: Tetsuji Okamoto

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Chen, KL., Wang, Y., Lin, ZP. et al. The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress. In Vitro Cell.Dev.Biol.-Animal 56, 635–641 (2020). https://doi.org/10.1007/s11626-020-00498-7

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  • DOI: https://doi.org/10.1007/s11626-020-00498-7

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