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Resveratrol promotes the embryonic development of vitrified mouse oocytes after in vitro fertilization

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Abstract

Vitrification of oocytes is closely associated with the lower embryonic developmental potential, which involves the cryopreservation injury occurred during vitrification. It indicates that vitrification may need to be further optimized. Therefore, we studied the effects of resveratrol, an antioxidant, on the developmental potential of vitrified mouse oocytes after in vitro fertilization. After adding a series of concentrations of resveratrol (0, 1, 10, 25, and 50 μM) into vitrification, warming, and post-warming mediums, we found that 25 and 50 μM resveratrol increased the blastocyst formation rate of vitrified oocytes. We further showed that 25 μM resveratrol increased the mean cell numbers of blastocyst from vitrified oocytes. 25 μM resveratrol reduced oxidative stress of vitrified oocytes through decreasing the levels of reactive oxygen species (ROS) and increasing the levels of glutathione (GSH), and 25 μM resveratrol alleviated the abnormal mitochondrial distribution pattern of oocytes after vitrification. In conclusion, our study implied that resveratrol could diminish the cryopreservation injuries during the vitrification of mouse oocytes and further confirmed that resveratrol may be an effective antioxidant to optimize vitrification.

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Acknowledgements

This work was supported in part by grants from the National Natural Science Foundation (81671413 and 81671414), Shanghai Municipal Education Commission—Gaofeng Clinical Medicine (20152510), and Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics (17DZ2271100).

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Author notes

  1. Yang Wang and Meiling Zhang contributed equally to this work.

Authors and Affiliations

  1. Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai, 200135, China

    Yang Wang, Meiling Zhang, Zi-Jiang Chen & Yanzhi Du

  2. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, 200135, China

    Yang Wang, Meiling Zhang, Zi-Jiang Chen & Yanzhi Du

  3. National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, 250021, China

    Zi-Jiang Chen

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  1. Yang Wang
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  2. Meiling Zhang
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  3. Zi-Jiang Chen
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  4. Yanzhi Du
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Correspondence to Yanzhi Du.

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Editor: Tetsuji Okamoto

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Wang, Y., Zhang, M., Chen, ZJ. et al. Resveratrol promotes the embryonic development of vitrified mouse oocytes after in vitro fertilization. In Vitro Cell.Dev.Biol.-Animal 54, 430–438 (2018). https://doi.org/10.1007/s11626-018-0262-6

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  • DOI: https://doi.org/10.1007/s11626-018-0262-6

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