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Evaluation of surfactants as solubilizing agents in microsomal metabolism reactions with lipophilic substrates

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Abstract

Solubilizing agents are routinely added when investigating the biotransformation of lipophilic substrates using hepatic microsomes. For highly lipophilic compounds, the concentration of solvent or surfactant necessary for dissolution can be detrimental to enzyme activity. This study evaluates the effect of 12 surfactants on microsomal metabolism and the ability of the same surfactants to improve the aqueous solubility of the pentabrominated diphenyl ether BDE-100, a lipophilic environmental contaminant previously found to be recalcitrant to in vitro metabolism. Of the surfactants investigated, Cremophor EL and Tween 80 displayed the best combination of increased BDE-100 solubility and minimal inhibition of microsomal metabolism. However, a comparison of the in vitro metabolism products of BDE-100 in the presence of the two surfactants revealed varying amounts of metabolites depending on the surfactant used.

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Acknowledgements

We thank Mr. Jim Stanford, California State University, East Bay, Hayward, CA for his assistance with the GC–MS. This work was supported by a California State University RSCA Faculty Grant to A.T.K.

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  1. Department of Chemistry and Biochemistry, California State University, East Bay, Hayward, CA, 94542, USA

    Kathleen Randall, Shun Wen Cheng & Anne Therese Kotchevar

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  1. Kathleen Randall
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  2. Shun Wen Cheng
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  3. Anne Therese Kotchevar
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Correspondence to Anne Therese Kotchevar.

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Editor: T. Okamoto

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Randall, K., Cheng, S.W. & Kotchevar, A.T. Evaluation of surfactants as solubilizing agents in microsomal metabolism reactions with lipophilic substrates. In Vitro Cell.Dev.Biol.-Animal 47, 631–639 (2011). https://doi.org/10.1007/s11626-011-9449-9

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  • DOI: https://doi.org/10.1007/s11626-011-9449-9

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