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MicroRNA-34a regulates high glucose-induced apoptosis in H9c2 cardiomyocytes

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Summary

Hyperglycemia is an important initiator of cardiovascular disease, contributing to the development of cardiomyocyte death and diabetic complications. The purpose of the present study was to investigate whether high glucose state could induce apoptosis of rat cardiomyocyte cell line H9c2 through microRNA-mediated Bcl-2 signaling pathway. The expression of miR-34a and Bcl-2 mRNA was detected by using real-time PCR. Western blotting was used to examine the changes in apoptosis-associated protein Bcl-2. Apoptosis of H9c2 cells was tested by using flow cytometry. The results showed that the expression of miR-34a was significantly elevated and that of Bcl-2 was strongly reduced, and apoptosis of cardiomyocytes was apparently increased in the high-glucose-treated H9c2 cells as compared with normal-glucose-treated controls. In addition, we identified Bcl-2 gene was the target of miR-34a. miR-34a mimics reduced the expression of Bcl-2 and increased glucose-induced apoptosis, but miR-34a inhibitor acted as the opposite mediator. Our data demonstrate that miR-34a contributes to high glucose-induced decreases in Bcl-2 expression and subsequent cardiomyocyte apoptosis.

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Authors and Affiliations

  1. Department of Cardiovascular Diseases, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Fang Zhao  (赵 芳), Yin-zhi Wei  (卫银芝), Bin Zhou  (周 斌), Han Wang  (汪 瀚), Ming Chen  (陈 明), Xue-dong Gan  (干学东) & Shi-xi Xiong  (熊世熙)

  2. Department of Oral Radiology, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China

    Bo Li  (李 波)

  3. Department of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Zhao-hui Wang  (汪朝晖)

Authors
  1. Fang Zhao  (赵 芳)
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  2. Bo Li  (李 波)
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  3. Yin-zhi Wei  (卫银芝)
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  4. Bin Zhou  (周 斌)
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  5. Han Wang  (汪 瀚)
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  6. Ming Chen  (陈 明)
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  7. Xue-dong Gan  (干学东)
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  8. Zhao-hui Wang  (汪朝晖)
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  9. Shi-xi Xiong  (熊世熙)
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Corresponding author

Correspondence to Fang Zhao  (赵 芳).

Additional information

This project was supported by grants from Hubei Natural Science Foundation of China (No. 2012FFB04307), Wuhan University Independent Research Projects Foundation (No. 303274034), Zhongnan Hospital of Wuhan University Hospital Foundation (No.201103), and National Natural Science Foundation of China (No. 390971103).

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Zhao, F., Li, B., Wei, Yz. et al. MicroRNA-34a regulates high glucose-induced apoptosis in H9c2 cardiomyocytes. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 834–839 (2013). https://doi.org/10.1007/s11596-013-1207-7

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  • DOI: https://doi.org/10.1007/s11596-013-1207-7

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