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Molecular phylogenetic analysis shows that Amanita ponderosa and A. curtipes are distinct species

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Abstract

Amanita curtipes and A. ponderosa are two Mediterranean taxa sharing a number of morphological features as well as their habitat. Their synonymy or variety status has been proposed by several authors. To clarify this taxonomic issue we have sequenced the D1-D2 domains of the 28S rRNA gene as well as the complete ITS1-5.8S-ITS2 region of several specimens of the two species collected in Spain, and aligned these sequences with those from other Amanita species. Molecular phylogenetic analysis based on the two regions revealed that A. ponderosa and A. curtipes are clearly distinct species. The distribution of Amanita species in the phylogenetic trees was consistent with the division of the genus in subgenera and sections as proposed by previous authors. Sequences of A. ponderosa and A. curtipes were grouped in a monophyletic cluster together with other species of the section Amidella. However, A. ponderosa was closer to other species in the section, such as A. peckiana and A. volvata, than to A. curtipes. We also indicate the macromorphological characters that are most useful to reliably distinguish A. ponderosa and A. curtipes.

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References

  • Bas C (1969) Morphology and subdivision of Amanita and a monograph on its section Lepidella. Persoonia 5:285–579

    Google Scholar 

  • Bridge PD, Roberts PJ, Spooner BM, Panchal G (2003) On the unreliability of published DNA sequences. New Phytol 160:43–48

    Article  CAS  Google Scholar 

  • Castro ML (1997) Analyse critique des taxons appartenant au groupe Amanita curtipes Gilbert (Agaricales, Basidiomycotina). Distribution dans la Peninsule Iberique. Doc Mycol 27:43–51

    Google Scholar 

  • Courtecuisse R, Duhem B (1994) Guide des champignons de France et d’Europe. Delachaux et Niestlé, Lausanne

    Google Scholar 

  • Daza A, Romero L, Santamaría C, Camacho M, Aguilar A, Megías M, Moreno G, Pérez M, Manjón JL (2002) El gurumelo, una seta de primavera. Quercus 193:20–24

    Google Scholar 

  • Daza A, Moreno G, Santamaría C, Romero de la Osa L, Bernedo M, Manjón JL (2003) Amanita ponderosa “Gurumelo”, un hongo a estudiar por su interés gastronómico-silvícola en Andalucía (Sierra de Aracena), provincia de Huelva. Bol Soc Micol Madr 27:91–99

    Google Scholar 

  • Drehmel D, Moncalvo JM, Vilgalys R (1999) Molecular phylogeny of Amanita based on large-subunit ribosomal DNA sequences: implications for taxonomy and character evolution. Mycologia 91:610–618

    Article  CAS  Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specifity for basidiomycetes. Aplication to the identification of mycorrhizae and rust. Mol Ecol 2:113–118

    PubMed  CAS  Google Scholar 

  • González V, Arenal F, Platas G, Esteve-Raventós F, Peláez F (2002) Molecular typing of Spanish species of Amanita by restriction analysis of the ITS region of the DNA. Mycol Res 106:903–910

    Article  CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hopple JS Jr, Vilgalys R (1994) Phylogenetic relationships among coprinoid taxa and allies based on data from restriction site mapping of nuclear rDNA. Mycologia 86:96–107

    Article  CAS  Google Scholar 

  • Konrad P, Maublanc A (1948) Les Agaricales vol 1. Encycl Mycol 14:1–464

    Google Scholar 

  • Kühner R, Romagnesi H (1953) Flore analitique des champignons superieurs. Masson, Paris

    Google Scholar 

  • Lim YW, Jung HS (1998) Phylogenetic relationships of Amanita species based on ITS1-5.8S rDNA-ITS2 region sequences. J Microbiol 36:203–207

    CAS  Google Scholar 

  • Malençon G, Heim R (1942) Notes critiques sur quelques hymènomycetes d’Europe et d’Afrique du Nord I. Les amanites blanches meridionales. Bull Soc Mycol Fr 58:14–34

    Google Scholar 

  • Mesplède VH (1980) Revision des Amanites. Bull Soc Mycol Bearn, num spec 50

  • Moncalvo JM, Drehmel D, Vilgalys R (2000) Variation in modes and rates of evolution in nuclear and mitochondrial ribosomal DNA in the mushroom genus Amanita (Agaricales, Basidiomycota): phylogenetic implications. Mol Phylogenet Evol 16:48–63

    Article  PubMed  CAS  Google Scholar 

  • Moncalvo JM, Vilgalys R, Redhead SA, Johnson JE, James TY, Aime MC, Hofstetter V, Verduin SJW, Larsson E, Baroni TJ, Thorn RG, Jacobsson S, Clémençon H, Miller OK Jr (2002) One hundred and seventeen clades of euagarics. Mol Phylogenet Evol 23:357–400

    Article  PubMed  CAS  Google Scholar 

  • Moreno G, Esteve-Raventós F (1988) Estudios micológicos en el Parque Natural de Monfragüe (Extremadura, España), I. Agaricales. Bol Soc Micol Madr 12:67–83

    Google Scholar 

  • Nicholas KB, Nicholas HB Jr, Deerfield DW (1997) GeneDoc: Analysis and Visualization of Genetic Variation. EMBNEW NEWS 4:14

    Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Evolutionary Biology Centre, Uppsala University

    Google Scholar 

  • Oda T, Tanaka C, Tsuda M (1999) Molecular phylogeny of Japanese Amanita species based on nucleotide sequences of the internal transcribed spacer region of nuclear ribosomal DNA. Mycoscience 40:57–64

    Article  CAS  Google Scholar 

  • O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds). The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB international, Wallingford, U.K., pp. 225–233

    Google Scholar 

  • Peláez F, Platas G, Collado J, Díez MT (1996) Infraspecific variation in two species of aquatic hyphomycetes, assessed by RAPD analysis. Mycol Res 100:831–837

    Article  Google Scholar 

  • Pinho-Almeida F (1994) Estudos taxonómicos no género Amanita. Secçao Amidella- Complexo Lepiotoides: A. curtipes Gilbert, A. lepiotoides Barla e A. ponderosa Malençon & Heim. Rev Biol (Lisboa) 15:131–151

    Google Scholar 

  • Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  PubMed  CAS  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  PubMed  CAS  Google Scholar 

  • Singer R (1986) The agaricales in modern taxonomy, 4th ed. Koeltz Scientific Books, Koenigstein, Germany, p 981

    Google Scholar 

  • Swofford DL (2001) PAUP*: phylogenetic analysis using parsimony (and other methods). Version 4.0b10. Sinauer, Sunderland, Mass.

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Weiß M, Yang ZL, Oberwinkler F (1998) Molecular phylogenetic studies in the genus Amanita. Can J Bot 76:1170–1179

    Article  Google Scholar 

  • Yang ZL (1997) Die Amanita-Arten von Suedwestchina. Bibl Mycol 170:1–240

    Google Scholar 

  • Zhang LF, Yang JB, Yang ZL (2004) Molecular phylogeny of eastern Asia species of Amanita (Agaricales, Basidiomycota): taxonomic and biogeographic implications. Fungal Divers 17:219–238

    Google Scholar 

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Acknowledgements

The authors would like to thank Dr. R. Korf for help with the English text. This study was made possible thanks to financial support from the Spanish Plan Nacional I+D of Research, projects 1FD97-1441 and SC00-081.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain

    Gabriel Moreno, Marieta Bernedo, Alba Vargas & José Luis Manjón

  2. Centro de Investigación Básica de España, Merck, Sharp & Dohme de España S.A., Josefa Valcárcel 38, Madrid, 28027, Spain

    Gonzalo Platas & Fernando Peláez

  3. IFAPA Centro “Las Torres-Tomejil”, Junta de Andalucía. Apartado oficial, 41200, Alcalá del Río (Sevilla), Spain

    Antonio Daza, Carmen Santamaría, María Camacho & Luis Romero de la Osa

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  1. Gabriel Moreno
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  2. Gonzalo Platas
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  3. Fernando Peláez
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  4. Marieta Bernedo
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  6. Antonio Daza
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  9. Luis Romero de la Osa
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  10. José Luis Manjón
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Correspondence to José Luis Manjón.

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Moreno, G., Platas, G., Peláez, F. et al. Molecular phylogenetic analysis shows that Amanita ponderosa and A. curtipes are distinct species. Mycol Progress 7, 41–47 (2008). https://doi.org/10.1007/s11557-007-0551-z

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  • DOI: https://doi.org/10.1007/s11557-007-0551-z

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