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Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate

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Abstract

This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-l-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P < 0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype.

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Acknowledgements

This study was supported, by grants from Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan (98TMU-WFH-08), and, in part, by Association for Dental Sciences, ROC, Taiwan.

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Authors and Affiliations

  1. School of Dentistry, Taipei Medical University, Taipei, Taiwan, ROC

    Sheng-Wei Feng, Wei-Jen Chang, Che-Tong Lin & Sheng-Yang Lee

  2. Dental Department of Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan, ROC

    Yi-June Lo

  3. Department of Biochemistry, School of Dentistry at Matsudo, Nihon University, Chiba, Japan

    Yoshimitsu Abiko

  4. Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan, ROC

    Haw-Ming Huang

  5. Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei, Taiwan, ROC

    Haw-Ming Huang

  6. Research Center for Biomedical Devices, Taipei Medical University, Taipei, Taiwan, ROC

    Haw-Ming Huang

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  1. Sheng-Wei Feng
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  2. Yi-June Lo
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  3. Wei-Jen Chang
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  4. Che-Tong Lin
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  7. Haw-Ming Huang
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Correspondence to Haw-Ming Huang.

Additional information

Sheng-Wei Feng, Yi-June Lo, and Wei-Jen Chang contributed equally to this study.

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Feng, SW., Lo, YJ., Chang, WJ. et al. Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-l-lactide substrate. Med Biol Eng Comput 48, 793–798 (2010). https://doi.org/10.1007/s11517-010-0639-5

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  • DOI: https://doi.org/10.1007/s11517-010-0639-5

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