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Fermentative production of dextran using Leuconostoc spp. isolated from fermented food products

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Frontiers in Biology

Abstract

Leuconostoc spp. (LS1and LI1) isolated from sauerkraut and idli batter was selected for dextran production. To enhance the yield of dextran, effects of various parameters such as sucrose concentration, pH, temperature, incubation and inoculum percentage were analyzed. The optimum sucrose concentration for the Leuconostoc spp. (LS1 and LI1) was found to be 15% and 25% respectively. Isolates produced maximum dextran after 20 h of incubation at 29° C and the optimum pH was found between 8 and 8.5. The inoculum concentration of 7.5% was more favorable for the production of dextran by Leuconostoc spp. (LS1 and LI1). The growth kinetic parameters were studied and compared for the strains LS1 and LI1. Mass production of dextran was carried out using a stirred tank batch reactor. FTIR analysis was done to determine the functional groups of dextran. sephadex is prepared by cross linking dextran using epichlorohydrin and the functional groups are determined by FTIR analysis.

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References

  • Alsop R M (1983). Industrial production of dextrans. Prog Ind Microbiol, 18: 1–42

    CAS  Google Scholar 

  • Aman A, Siddiqui N N, Shah A U Q (2011). Characterization and potential applications of high molecular weight dextran produced by Leuconostoc mesenteroides AA1. Carbohydr Polym, 87(1): 910–915

    Article  Google Scholar 

  • Güner A, Akman Ö, Rzaev Z M O (2001). CCrosslinking of dextran with some selective Cl-, P- and N-containing functional substances in aqueous solutions. React Funct Polym, 47(1): 55–65

    Article  Google Scholar 

  • Halasz H, Barath A, Holzapfel W H (1999). The influence of starter culture selection on sauerkraut fermentation. Z Lebensm Unters Forsch, 208(5–6): 434–438

    CAS  Google Scholar 

  • Hamasaki Y, Ayaki M, Fuchu H, Sugiyama M, Morita H (2003). Behaviour of psychrotrophic lactic acid bacteria isolated from spoiling cooked meat products. Appl Environ Microbiol, 69(6): 3668–3671

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Holt J G (1994). Group 17 Gram-Positive Cocci: Bergey’s Manual of Determinative Bacteriology, ed 9th. Baltimore: William & Wilkins: 529–541

    Google Scholar 

  • Kaboli H, Reilly P (1980). Immobilization and properties of Leuconostoc mesenteroides dextransucrase. Biotechnol Bioeng, 22(5): 1055–1069

    Article  CAS  Google Scholar 

  • Katina K, Maina N H, Juvonen R, Flander L, Johansson L, Virkki L, Tenkanen M, Laitila A (2009). In situ production and analysis of Weissella confuse dextran in wheat sourdough. Food Microbiol, 26(7): 734–743

    Article  CAS  PubMed  Google Scholar 

  • Khan F, Khanam A, Parihar M S, Bilgainya R, Rai K, Khan F (2010). Dissipative convective structures and nanoparticles encapsulation in Cu/alginate/dextran composite hydrogels and sponges. Carbohydr Polym, 83(2): 586–590

    Article  Google Scholar 

  • Kim D, Day DF (1994) A New process for the production of clinical dextran by mixed culture fermentation of Lipomyces starkeyi and Leuconostoc mesenteroides, Enzyme Microbial Technol, 16: 844–848.

    Article  CAS  Google Scholar 

  • Kim D, Robyt J F, Lee S Y, Lee J H, Kim Y M (2003). Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of Leuconostoc mesenteroides B-512FMCM dextransucrase. Carbohydr Res, 338(11): 183–1189

    Article  Google Scholar 

  • Leathers T D, Hayman G T, Cote G L (1995). Rapid screening of Leuconostoc mesenteroides mutants for elevated proportions of alternan to dextran. Curr Microbiol, 31(1): 19–22

    Article  CAS  Google Scholar 

  • Martinez-Espindola J P, Lopez-Manguia C A (1985). On the kinetics of dextransucrase and dextran synthesis in batch reactors. Biotechnol Lett, 7(7): 483–486

    Article  CAS  Google Scholar 

  • Moosavi-Nasab M, Gavahian M, Yousefi A R. Hamed A (2010). Fermentative production of dextran using food industry wastes. World Acad Sci Eng Technol: 68

  • Santos M J, Teixeira J, Rodrigues A (2000). Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B512 (f). Biochem Eng J, 4(3): 177–188

    Article  CAS  Google Scholar 

  • Sarwat F, Shah A U Q, Aman A, Ahmed N (2008). Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713. Int J Biol Sci, 4: 379–386

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shah Ali UL Qader, Lubna Iqbal, Afsheen Aman, Erum Shireen, Abid Azhar (2005). Production of dextran by newly isolated strains of Leuconostoc mesenteroides PCSIR-4 and PCSIR-9. Turk J Biochem, 31(1): 21–26

    Google Scholar 

  • Sutherland I W (1996). Extracellular polysaccharides. Biotechnol, 6(2): 145

    Google Scholar 

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Authors and Affiliations

  1. School of Biosciences and Techndogy, VIT University, Vellore, 632014, Tamil Nadu, India

    C. Subathra Devi, Shantan Reddy & V. Mohanasrinivasan

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  1. C. Subathra Devi
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  2. Shantan Reddy
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  3. V. Mohanasrinivasan
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Correspondence to C. Subathra Devi.

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Subathra Devi, C., Reddy, S. & Mohanasrinivasan, V. Fermentative production of dextran using Leuconostoc spp. isolated from fermented food products. Front. Biol. 9, 244–253 (2014). https://doi.org/10.1007/s11515-014-1303-5

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  • DOI: https://doi.org/10.1007/s11515-014-1303-5

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