Abstract
Aqueous suspensions containing increasing amounts (0.1-200 g/L) of egg white at different ionic strength (0.025-1.000 M NaCl) were characterised for critical concentration associated to self crowding phenomena. Samples were then exposed to 30 W m− 2 UV-C light for 10 min. Self crowded proteins resulted fully resistant to photodegradation. In uncrowded conditions, egg white underwent fragmentation and aggregation. The increase in ionic strength favoured protein folding. Compact proteins were more prone to intramolecular rearrangement and reached photostability at higher concentrations. Self crowding could be selectively exploited to steer protein resistance to physical stresses.
Similar content being viewed by others
References
A.P. Minton, Biopolym. 20, 2093 (1981)
P. Walstra, Physical chemistry of foods (Marcel Dekker, Inc., Basel, 2003), pp. 137–248
R. Lapasin, S. Pricl, Rheology of industry polisaccharides: Theory and applications (Blackie Academic & Professional, Glasgow, 1995). Chapter 4
S.B. Zimmermann, S.O. Trach, J. Mol. Biol. 222, 599 (1991)
B. Van Den Berg, R. Wain, C.M. Dobson, R.J. Ellis, Eur. Mol. Biol. Organ. J 19, 3870 (2000)
F. Despa, D.P. Orgill, R.C. Lee, Ann. Biomed. Eng. 33(8), 1125–1131 (2005)
F. Despa, D.P. Orgill, R.C. Lee, Ann. N.Y. Acad. Sci. 1066, 54–66 (2005). 2005
J. Hong, L.M. Gierasch, J. Am. Chem. Soc. 132, 10445 (2010)
A.B. Fulton, Cell 30, 345 (1982)
A.P. Minton, Mol. Cell. Biochem. 55, 119 (1983)
R.J. Ellis, Trends Biochem. Sci. 26, 597 (2001)
L. Manzocco, M.C. Nicoli, Food Chem. 132, 982 (2012)
M.L. Huggins, J. Am. Chem. Soc. 64, 1712 (1942)
S.E. Harding, Prog. Biophys. Mol. Biol. 68, 207 (1997)
I.M. Krieger, M. Eguiluz, J. Rheol. 20, 29 (1976)
S. Yadav, S.J. Shire, D.S. Kalonia, Pharm. Res. 28, 1973 (2011)
S. Yadav, S.J. Shire, D.S. Kalonia, J. Pharm. Sci. 101, 998 (2012)
A. Einstein, Ann. Phys. 17, 549 (1905)
E.C.Y. Li-Chan, W.D. Powrie, S. Nakay, in Egg science and technology, ed. by W.J. Stadelman, O.J. Cotterill (The Haworth Press, Inc, New York, 1995), p. 105
A.C. Awadé, T. Efstathiou, J. Chromatogr. 723, 69 (1999)
M.J. Davies, R.J. Truscott, J. Photochem. Photobiol., B 63, 114 (2001)
M.J. Davies, Biochem. Biophys. Res. Comm. 305, 761 (2003)
L.Z. Wu, Y.B. Sheng, J.B. Xie, W. Wang, J. Mol. Struct. 882, 101 (2008)
L. Manzocco, A. Panozzo, M.C. Nicoli, Food Chem. 135, 522 (2012)
L. Manzocco, B. Quarta, A. Dri, Inn. Food Sci. Emerg. Technol. 10, 506 (2009)
J.D. Goosey, J.S. Zigler Jr., J.H. Kinoshita, Science 208, 1278 (1980)
J.W. Rhim, A. Gennadios, D. Fu, C.L. Weller, M.A. Hanna, Lebensm. Wiss. Technol. 32, 129 (1999)
S. Damodaran, in Fennema’s food chemistry, ed. by S. Damodaran, K.L. Parkin, O.R. Fennema, 4th edn. (CRC Press, Boca Raton, 2008), p. 247
J. Xie, M. Qin, Y. Cao, W. Wang, Proteins 79, 2505 (2011)
K.W. Minton, P. Karmin, G.M. Hahn, A.P. Minton, Proc. Natl. Acad. Sci. U. S. A. 79, 7107 (1982)
B. van den Berg, R.J. Ellis, C.M. Dobson, Eur. Mol. Biol. Organ. J. 18, 6927 (1999)
R.J. Ellis, A.P. Minton, Biol. Chem. 387, 485 (2006)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Manzocco, L., Nicoli, M.C. Self Crowding as a Determinant of egg white Photostability. Food Biophysics 10, 155–161 (2015). https://doi.org/10.1007/s11483-014-9377-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11483-014-9377-9