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Preparation of bovine hemoglobin-imprinted polymer beads via the photografting surface-modified method

  • Research Article
  • Published:
Frontiers of Chemistry in China

Abstract

Molecularly imprinted polymers (MIPs), based on photografting surface-modified polystyrene beads as matrices, were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N′-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope (SEM) and elemental analyses demonstrated the formation of a grafting polymer layer on the polystyrene-bead surface. Subsequent removal of the template left behind cavities on the surface of the polymer matrix with a shape and an arrangement of functional groups having complementary binding sites with the original template molecule. The adsorption studies showed that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via the photografting surface-modified method exhibited better selectivity for the template. Attempts to employ the new method in molecular imprinting techniques may introduce new applications for MIPs and facilitate probable protein separation and purification.

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Authors and Affiliations

  1. Department of Chemistry, Nankai University, Tianjin, 300071, China

    Qingqing Gai, Qiuye Liu, Wenyou Li, Xiwen He, Langxing Chen & Yukui Zhang

  2. Changzhi Medical College, Changzhi, 046000, China

    Qingqing Gai

Authors
  1. Qingqing Gai
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  2. Qiuye Liu
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  3. Wenyou Li
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  4. Xiwen He
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  5. Langxing Chen
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  6. Yukui Zhang
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Correspondence to Yukui Zhang.

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Translated from Chemical Journal of Chinese Universities, 2008, 29(1): 64–70

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Gai, Q., Liu, Q., Li, W. et al. Preparation of bovine hemoglobin-imprinted polymer beads via the photografting surface-modified method. Front. Chem. China 3, 370–377 (2008). https://doi.org/10.1007/s11458-008-0089-x

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  • DOI: https://doi.org/10.1007/s11458-008-0089-x

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