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Polymorphism of human cytomegalovirus (HCMV) UL144 gene in low passage clinical isolates

  • Article
  • Virology
  • Published:
Chinese Science Bulletin

Abstract

To explore the impact of gene polymorphism of human cytomegalovirus (HCMV) on virus virulence, the full-length UL144 gene and partial sequence of glycoprotein B (UL55) gene were sequenced and analyzed for 23 clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection. Among the 23 isolates, 13 (57 %) were UL144 genotype 1A, 3(13 %) were UL144 genotypes 2 and 7(30 %) were UL144 genotype 3; geographic differences in genotype distribution were found for both UL144 and gB gene. No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US. Our results also demonstrated that for all clinical strains of gB genotypes I, III and UL144 genotypes 1A, 2, and 3 found in this study, mother-to-fetus vertical transmission was possible.

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Acknowledgements

This work was supported by the Innovation Platform Open Funds for Universities in Hunan Province (09K022 and 11K010), the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Shanghai Institute of Biological Products, Shanghai, 200052, China

    Xingxing Fu & Ze Chen

  2. Institute of Pediatric Research, Hunan Children’s Hospital, Changsha, 410007, China

    Liping Li

  3. Laboratory of Molecular Virology, School of Life Science, Hunan Normal University, Changsha, 410084, China

    Chaoyang Huang, Xiaoman Li & Ze Chen

  4. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Huadong Wang & Ze Chen

  5. Xie Tu Community Medical Service Center, Shanghai, 200032, China

    Zhenyuan Xie

Authors
  1. Xingxing Fu
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  2. Liping Li
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  3. Chaoyang Huang
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  4. Xiaoman Li
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  5. Huadong Wang
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  6. Zhenyuan Xie
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  7. Ze Chen
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Corresponding authors

Correspondence to Zhenyuan Xie or Ze Chen.

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Fu, X., Li, L., Huang, C. et al. Polymorphism of human cytomegalovirus (HCMV) UL144 gene in low passage clinical isolates. Chin. Sci. Bull. 59, 3120–3126 (2014). https://doi.org/10.1007/s11434-014-0353-z

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  • DOI: https://doi.org/10.1007/s11434-014-0353-z

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