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The c-myc coding DNA sequences of cyprinids (Teleostei: Cypriniformes): Implications for phylogeny

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  • Zoology
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Chinese Science Bulletin

Abstract

The family Cyprinidae is one of the largest fish families in the world, which is widely distributed in East Asian, with obvious difference in characteristic size among species. The phylogenetic analysis of cyprinid taxa based on the functionally important genes can help to understand the speciation and functional divergence of the Cyprinidae. The c-myc gene is an important gene regulating individual growth. In the present study, the sequence variations of the cyprinid c-myc gene and their phylogenetic significance were analyzed. The 41 complete sequences of the c-myc gene were obtained from cyprinids and outgroups through PCR amplification and clone. The coding DNA sequences of the c-myc gene were used to infer molecular phylogenetic relationships within the Cyprinidae. Myxocyprinus asiaticus (Catostomidae), Misgurnus anguillicaudatus (Cobitidae) and Hemimyzon sinensis (Homalopteridae) were assigned to the outgroup taxa. Phylogenetic analyses using maximum parsimony (MP), maximum likelihood (ML), and Bayesian retrieved similar topology. Within the Cyprinidae, Leuciscini and Barbini formed the monophyletic lineage respectively with high nodal supports. Leuciscini comprises Xenocyprinae, Cultrinae, East Asian species of Leuciscinae and Danioninae, Gobioninae and Acheilognathinae, and Barbini contains Schizothoracinae, Barbinae, Cyprininae and Labeoninae. Danio rerio, D. myersi and Rasbora trilineata were supposed to separate from Leuciscinae and Barbini and to form another lineage. The positions of some Danioninae species were still unresolved. Analyses of both amino acid variation with parsimony information and two high variation regions indicated that there is no correlation between variations of single amino acid or high variation regions and characteristic size of cyprinids. In addition, the species with smaller size were usually found to be basal within clades in the tree, which might be the results of the adaptation to the primitive ecology and survival pressure.

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References

  1. Chen Y Y. Fauna Sinica, Osteichthyes, Cypriniformes (II) (in Chinese). Beijing: Science Press, 1998. 1

    Google Scholar 

  2. Nelson J S. The Fish of the World. 3rd ed. New York: Wiley, 1995. 1–18

    Google Scholar 

  3. Chen X L, Le P Q, Lin R D. Major groups within the family Cyprinidae and their phylogenetic relationships. Acta Zootaxon Sin (in Chinese), 1984, 9(4): 424–440

    Google Scholar 

  4. Howes G J. Systematics and biogeography: An overview. In: Winfield I J, Nelson J S, eds. Cyprinid Fishes: Systematics, Biology and Exploitation. London: Chapman and Hall, 1991. 1–33

    Google Scholar 

  5. Cavender T, Coburn M. Phylogenetic relationships of North American Cyprinidae. In: Mayden R, ed. Systematics Historical Ecology, and North American Freshwater Fishes. Standford: Standford University Press, 1992. 293–327

    Google Scholar 

  6. Briolay J, Galtier N, Brito R M, et al. Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Mol Phylogenet Evol, 1998, 9(1): 100–108

    Article  Google Scholar 

  7. Gilles A, Lecointre G, Faure E, et al. Mitochondrial phylogeny of the European cyprinids: Implications for their systematics, reticulate evolution and colonization time. Mol Phylogenet Evol, 1998, 10(1):132–143

    Article  Google Scholar 

  8. Gilles A, Lecointre G, Miguelis A, et al. Partial combination applied to phylogeny of European cyprinids using the mitochondrial control region. Mol Phylogenet Evol, 2001, 19: 22–33

    Article  Google Scholar 

  9. Zardoya R. Molecular evidence on the evolutionary and Biogeographical pattern of European cyprinids. J Mol Evol, 1999, 49:227–237

    Article  Google Scholar 

  10. Simons A M, Mayden R L. Phylogenetic relationships of the creek chubs and the spinefins: An enigmatic group of north American cyprinid fishes (Actinopterygii: Cyprinidae). Cladistics, 1997, 13:187–205

    Article  Google Scholar 

  11. Simons A M, Mayden R L. Phylogenetic relationships of the western North American phoxinins (Actinopterygii: Cyprinidae) as inferred from mitochondrial 12S and 16S ribosomal RNA sequences. Mol Phylogenet Evol, 1998, 9: 308–329

    Article  Google Scholar 

  12. Simons A M, Mayden R L. Phylogenetic relationships of North American cyprinids and assessment of homology of the openposterior myodome. Copeia, 1999, 1: 13–21

    Article  Google Scholar 

  13. Xiao W, Zhang Y, Liu H. Molecular systematics of xenocyprinae (Teleostei: Cyprinidae): Taxonomy, biogeography and coevolution of a special group restricted in East Asia. Mol Phylogenet Evol, 2001, 18:163–173

    Article  Google Scholar 

  14. He S, Chen Y, Nakajima T. Sequences of cytochrome b gene for primitive cyprinid fishes in East Asia and their phylogenetic concerning. Chin Sci Bull, 2001, 46(8): 661–665

    Article  Google Scholar 

  15. He S, Liu H, Chen Y, et al. Molecular phylogenetic relationships of Eastern Asian Cyprinidae (Pisces: Cyprinidformes) inferred from cytochrome b sequences. Sci China Ser C-Life Sci, 2004, 47(2):130–138

    Article  Google Scholar 

  16. Wang X, He S, Chen Y. Sequence variations of the S7 ribosomal protein gene in primitive cyprinid fishes: Implication on phylogenetic analysis. Chin Sci Bull, 2002, 47(19): 1638–1643.

    Article  Google Scholar 

  17. Wang X, Liu H, He S, et al. Sequence analysis of cytochrome b gene indicated that East Asian group of cyprinid subfamily Leuciscinae (Teleostei: Cyprinidae) evolved independently. Prog Natl Sci, 2004, 14(2): 132–137

    Article  Google Scholar 

  18. Atchley W R, Fitch W M. Myc and Max: Molecular evolution of a family of proto-oncogene products and their dimerization partner. Proc Natl Acad Sci USA, 1995, 92: 10217–10221

    Article  Google Scholar 

  19. Marcu K B, Bossone S A, Patel A J. Myc function and regulation. Annu Rev Biochem, 1992, 61: 809–861

    Article  Google Scholar 

  20. Johnston L A, Prober D A, Edgar B A, et al. Drosophila myc regulates cellular growth during development. Cell, 1999, 98: 779–790

    Article  Google Scholar 

  21. Trumpp A, Refaeli Y, Oskarsson T, et al. c-myc regulates mammalian body size by controlling cell number but not cell size. Nature, 2001, 414: 768–773

    Article  Google Scholar 

  22. Schreiber-Agus N, Stein D, Chen K, et al. Drosophila Myc is oncogenic in mammalian cells and plays a role in the diminutive phenotype. Proc Natl Acad Sci USA, 1997, 94: 1235–1240

    Article  Google Scholar 

  23. Prendergast G C. Myc structure and function. In: Yaniv M, Ghysdael J, eds. Oncogenes As Transcriptional Regulators. Vol 1. Basel: Birkhauser Verlag, 1997. 1–28

    Google Scholar 

  24. Steppan S J, Storz B L, Hoffmann R S. Nuclear DNA phylogeny of the squirrels (Mammalia: Rodentia) and the evolution of arboreality from c-myc and RAG1. Mol Phylogenet Evol, 2004, 30: 703–719

    Article  Google Scholar 

  25. Irestedt M, Johansson U S, Parsons T J, et al. Phylogeny of major lineages of suboscines (Passeriformes) analysed by nuclear DNA sequence data. J Avian Biol, 2001, 32: 15–25

    Article  Google Scholar 

  26. Harshman J, Huddleston C J, Bollback J P, et al. True and false gavials: A nuclear gene phylogeny of Crocodylia. Syst Biol, 2003, 52:386–402

    Article  Google Scholar 

  27. Crawford A J, Smith E N. Cenozoic biogeography and evolution in direct-developing frogs of Central America (Leptodactylidae: Eleutherodactylus) as inferred from a phylogenetic analysis of nuclear and mitochondrial genes. Mol Phylogenet Evol, 2005, 35:536–555

    Article  Google Scholar 

  28. Sambrook J, Fritsch E, Maniatis T. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1989

    Google Scholar 

  29. Thompson J D, Gibson T J, Plewniak F, et al. The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 1997, 25: 4876–4882

    Article  Google Scholar 

  30. Kumar S, Tamura K, Nei M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform, 2004, 5: 150–163

    Article  Google Scholar 

  31. Xia X, Xie Z. DAMBE: Data analysis in molecular biology and evolution. J Hered, 2001, 92: 371–373

    Article  Google Scholar 

  32. Posada D, Crandall K A. Modeltest: Testing the model of DNA substitution. Bioinformatics, 1998, 14(9): 817–818

    Article  Google Scholar 

  33. Swofford D L. PAUP: Phylogenetic analysis using Parsimony, Version4. Sunderland: Sinauer Associates, 2002.

    Google Scholar 

  34. Huelsenbeck J P, Ronquist F. MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 2001, 17: 754–755

    Article  Google Scholar 

  35. Ronquist F, Huelsenbeck J P. MRBAYES 3: Bayesian phylogenetic inference under mixed model. Bioinformatics, 2003, 19:1572–1574

    Article  Google Scholar 

  36. Schreiber-agus N, Horner J, Torres R, et al. Zebra fish myc family and max genes: Differential expression and oncogenic activity throughout vertebrate evolution. Mol Cell Biol, 1993, 13: 2765–2277

    Google Scholar 

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Authors and Affiliations

  1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Kong XiangHui, Wang XuZhen, Gan XiaoNi & He ShunPing

  2. College of Life Science, Henan Normal University, Xinxiang, 453007, China

    Kong XiangHui

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  1. Kong XiangHui
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  2. Wang XuZhen
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  3. Gan XiaoNi
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  4. He ShunPing
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Correspondence to He ShunPing.

Additional information

Supported by the Development Plan of the State Key Fundamental Research of China (Grant No. 2004CB117402), the National Natural Science Key Foundation of China (Grant No. 30530120) and Chinese Postdoctoral Science Foundation (Grant No. 2005037684)

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Kong, X., Wang, X., Gan, X. et al. The c-myc coding DNA sequences of cyprinids (Teleostei: Cypriniformes): Implications for phylogeny. CHINESE SCI BULL 52, 1491–1500 (2007). https://doi.org/10.1007/s11434-007-0216-y

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  • DOI: https://doi.org/10.1007/s11434-007-0216-y

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